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DVL1 Antibody

DVL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27384-1-AP

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, NIH/3T3 cells, mouse cerebellum tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 27384-1-AP immunogen is Fusion Protein DVL1 protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DVL1 fusion protein Ag26055
Full Name dishevelled, dsh homolog 1 (Drosophila)
Calculated molecular weight 75 kDa
Observed molecular weight 75 kDa, 73 kDa
GenBank accession number BC050454
Gene symbol DVL1
Gene ID (NCBI) 1855
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1–3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

Protocols

Product Specific Protocols
WB protocol for DVL1 antibody 27384-1-APDownload protocol
IHC protocol for DVL1 antibody 27384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Obstet Gynaecol

cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro.

Authors - Yasushi Yamada
ratWB

Neurosci Lett

Dishevelled-1 regulated apoptosis through NF-κB in cerebral ischemia/reperfusion injury in rats.

Authors - Xiaogang Zhang
  • KD Validated
humanIHC

Theranostics

Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/β-catenin pathway in hepatocellular carcinoma.

Authors - Junwei Song