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  • KD/KO Validated

DVL1 Polyclonal antibody

DVL1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27384-1-AP

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1



Tested Applications

Positive WB detected inMCF-7 cells, NIH/3T3 cells, mouse cerebellum tissue, HEK-293 cells, MDA-MB-231 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27384-1-AP targets DVL1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DVL1 fusion protein Ag26055
Full Name dishevelled, dsh homolog 1 (Drosophila)
Calculated Molecular Weight 75 kDa
Observed Molecular Weight75 kDa, 73 kDa
GenBank Accession NumberBC050454
Gene Symbol DVL1
Gene ID (NCBI) 1855
RRIDAB_2880859
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

Protocols

Product Specific Protocols
WB protocol for DVL1 antibody 27384-1-APDownload protocol
IHC protocol for DVL1 antibody 27384-1-APDownload protocol
IF protocol for DVL1 antibody 27384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Theranostics

Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/β-catenin pathway in hepatocellular carcinoma.

Authors - Junwei Song
mouseWB

Brain Res Bull

Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.

Authors - Qian Wu
ratWB

Neural Plast

Lycopene-Loaded Microemulsion Regulates Neurogenesis in Rats with Aβ-Induced Alzheimer's Disease Rats Based on the Wnt/β-catenin Pathway.

Authors - Wen-Jing Ning
mouseWB

Biol Trace Elem Res

Selenium Deficiency Leads to Changes in Renal Fibrosis Marker Proteins and Wnt/β-Catenin Signaling Pathway Components.

Authors - Tingting Lin
ratWB

Neurosci Lett

Dishevelled-1 regulated apoptosis through NF-κB in cerebral ischemia/reperfusion injury in rats.

Authors - Xiaogang Zhang
  • KD Validated
humanIHC

J Obstet Gynaecol

cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro.

Authors - Yasushi Yamada