Tested Applications
| Positive IHC detected in | rat liver tissue, human placenta tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, NIH/3T3 cells, C6 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive Dot Blot detected in | peptide |
| Positive ChIP-qPCR detected in | HeLa cells |
This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84702-1-RR targets Di-Methyl-Histone H3 (Arg2) in IHC, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | histone cluster 1, H3a |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_3672118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Arginine dimethylation is an important post-translational modification of proteins. Based on the positions of the two methyl groups on the guanidino group of arginine, it can be divided into symmetric dimethylation (SDMA) and asymmetric dimethylation (ADMA). Symmetric dimethylation is crucial for embryonic development and cell differentiation and is often associated with transcriptional repression. This antibody specifically recognizes symmetric dimethyl-arginine (SDMA, Rme2s).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RR | Download protocol |
| IHC protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Di-Methyl-Histone H3 (Arg2) Recombinant monoclonal antibody (catalog number 84702-1-RR) has 1 total citation. It appears in the journal International Dental Journal (Int Dent J), in a study on PRMT6-regulated odontogenic differentiation and mitochondrial function of stem cells from apical papilla via the β-catenin pathway. The research field is epigenetics/histone modification, applied in ChIP for human samples.
| Species | Application | Title |
|---|---|---|
Int Dent J Protein Arginine Methyltransferase 6 Regulated Odontogenic Differentiation and Mitochondrial Function of Stem Cells from Apical Papilla Through its Nuclear Localization Sequence via β-catenin Pathway. |
