Di-Methyl-Histone H3 (Arg2) Recombinant monoclonal antibody, PBS Only

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  IHC staining of human placenta using 84702-1-RR (same clone as 84702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 84702-1-RR (same clone as 84702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84702-1-RR (same clone as 84702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84702-1-RR (same clone as 84702-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84702-1-RR (same clone as 84702-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 84702-1-RR (same clone as 84702-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C6 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84702-1-RR (same clone as 84702-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  Dot Blot experiment of peptide using 84702-1-RR (same clone as 84702-1-PBS)

  Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

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  ChIP experiment of HeLa using 84702-1-RR (same clone as 84702-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Di-Methyl-Histone H3 (Arg2) (84702-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84702-1-PBS in a different storage buffer formulation.

Di-Methyl-Histone H3 (Arg2) Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR

Cat No. 84702-1-PBS

Clone No.241896D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR

Synonyms

Di Methyl Histone H3, Di-Methyl Histone H3 (Arg2), Di-Methyl Histone H3 Arg2, Di-Methyl Histone-H3 Arg2, Di-Methyl-Histone H3

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Product Information

84702-1-PBS targets Di-Methyl-Histone H3 (Arg2) in IHC, IF/ICC, FC (Intra), Dot Blot, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: histone cluster 1, H3a
• GenBank Accession Number: BC066245
• Gene Symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P68431
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Arginine dimethylation is an important post-translational modification of proteins. Based on the positions of the two methyl groups on the guanidino group of arginine, it can be divided into symmetric dimethylation (SDMA) and asymmetric dimethylation (ADMA). Symmetric dimethylation is crucial for embryonic development and cell differentiation and is often associated with transcriptional repression. This antibody specifically recognizes symmetric dimethyl-arginine (SDMA, Rme2s).