Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, T-47D cells, HCT 116 cells |
| Positive IHC detected in | human colon cancer tissue, human ovary cancer tissue, human tonsillitis tissue, human stomach cancer tissue, human appendicitis tissue, human breast cancer tissue, human colon cancer tissue, human placenta tissue, human colorectal cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83991-4-RR targets E-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1112 Product name: Recombinant Human E-cadherin protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 155-709 aa of NM_004360.5 Sequence: DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPA Predict reactive species |
| Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
| Calculated Molecular Weight | 97 kDa |
| Observed Molecular Weight | 135 kDa |
| GenBank Accession Number | NM_004360.5 |
| Gene Symbol | E-cadherin |
| Gene ID (NCBI) | 999 |
| RRID | AB_3671566 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P12830-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for E-cadherin antibody 83991-4-RR | Download protocol |
| IHC protocol for E-cadherin antibody 83991-4-RR | Download protocol |
| WB protocol for E-cadherin antibody 83991-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The E-cadherin Recombinant monoclonal antibody (241098G6), catalog number 83991-4-RR, has 2 total citations. These appear in Biomedicines and Biochem Biophys Res Commun. The research fields include oral cancer ferroptosis (TPI1 inhibition sensitizing cisplatin-resistant cells) and pancreatic ductal adenocarcinoma (COP9 signalosome 8-mediated autophagy driving proliferation, invasion, and metastasis).
| Species | Application | Title |
|---|---|---|
Biomedicines Inhibition of TPI1 Sensitizes Cisplatin-Resistant Oral Cancer to Ferroptosis | ||
Biochem Biophys Res Commun COP9 signalosome 8 mediated autophagy drives proliferation, invasion, and metastasis in pancreatic ductal adenocarcinoma. |
