EEA1 Antibody

EEA1 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G8B2

Cat no : 66218-1-Ig

Synonyms

early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66218-1-Ig targets EEA1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name early endosome antigen 1
Calculated molecular weight 162 kDa
Observed molecular weight
GenBank accession number NM_003566
Gene symbol EEA1
Gene ID (NCBI) 8411
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic affinity chromatograph
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protocols

Product Specific Protocols
IF protocol for EEA1 antibody 66218-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

The Late Domain of Prototype Foamy Virus Gag Facilitates Autophagic Clearance of Stress Granules by Promoting Amphisome Formation.

Authors - Yingcheng Zheng
humanIF

Theranostics

Homotrimer cavin1 interacts with caveolin1 to facilitate tumor growth and activate microglia through extracellular vesicles in glioma.

Authors - Lin Wang
humanIF

J Clin Invest

TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation.

Authors - Haiqing Zhang