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EEA1 Antibody 0 Publications

Mouse Monoclonal| Catalog number: 66218-1-Ig |CloneNo.: 1G8B2

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Con: 117 μg/150 μl

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Tested Applications

Applications:
IF, ELISA
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EEA1 Antibody IF HeLa cells 66218-1-Ig

Immunofluorescent analysis of ( -20℃ Acetone) fixed HeLa cells using 66218-1-Ig (EEA1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
human
Positive IF detected in:
HeLa cells
Recommended dilution:
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Thiophilic affinity chromatograph
Isotype:
IgM
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
early endosome antigen 1
Calculated molecular weight:
162 kDa
Observed molecular weight:
GenBank accession number:
NM_003566
Gene ID (NCBI):
8411
Gene symbol
EEA1
Synonyms
early endosome antigen 1, EEA1, MST105, MSTP105, ZFYVE2

Background

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.


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