Tested Applications
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68065 targets EEA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28814 Product name: Recombinant human EEA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1151-1350 aa of NM_003566 Sequence: KLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWA Predict reactive species |
| Full Name | early endosome antigen 1 |
| Calculated Molecular Weight | 162 kDa |
| Observed Molecular Weight | 162 kDa |
| GenBank Accession Number | NM_003566 |
| Gene Symbol | EEA1 |
| Gene ID (NCBI) | 8411 |
| RRID | AB_2919561 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15075 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 EEA1 antibody CL488-68065 | Download protocol |
| IF protocol for CL Plus 488 EEA1 antibody CL488-68065 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL488-68065 is the EEA1 Monoclonal antibody (1E11E4), with a total of 5 citations. These appear in Nature, Autophagy, Advanced Science, Small Science, and Life Sciences. The associated research fields span virology (tick-borne encephalitis virus), renal medicine (macrophage autophagy in acute kidney injury), regenerative medicine (extracellular vesicle reprogramming for tissue repair), nanomedicine (chemotherapeutic nanocarriers in colorectal cancer), and immunology (macrophage phagocytic response to MRSA infection).
| Species | Application | Title |
|---|---|---|
Autophagy Macrophage autophagy protects against acute kidney injury by inhibiting renal inflammation through the degradation of TARM1 | ||
Adv Sci (Weinh) In Vivo Reprogramming of Tissue-Derived Extracellular Vesicles for Treating Chronic Tissue Injury Through Metabolic Engineering | ||
Small Sci Macropinocytic Uptake and pH-Responsive Endolysosomal Processing Drive Sustained Chemotherapeutic Efficacy of High-Load Core@Shell Nanocarriers in Colorectal Cancer. | ||
Life Sci Methylsulfonylmethane protects against lethal MRSA infection via binding to PIK3CB to increase macrophage phagocytic function and nitric oxide production. |
