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  • KD/KO Validated

EEF1A1 Polyclonal antibody

EEF1A1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 11402-1-AP

Synonyms

CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, GRAF 1EF, HNGC:16303, LENG7, PTI1



Tested Applications

Positive WB detected inHepG2 cells, mouse heart tissue, rat brain tissue, mouse ovary tissue, mouse liver tissue, human brain tissue, Jurkat cells, SH-SY5Y cells, mouse skeletal muscle tissue, mouse pancreas tissue, HeLa cells, multi-cells , MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11402-1-AP targets EEF1A1 in WB, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEF1A1 fusion protein Ag1938
Full Name eukaryotic translation elongation factor 1 alpha 1
Calculated molecular weight 462 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC014224
Gene symbol EEF1A1
Gene ID (NCBI) 1915
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell growth and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human EEF1A1.

Protocols

Product Specific Protocols
WB protocol for EEF1A1 antibody 11402-1-APDownload protocol
IHC protocol for EEF1A1 antibody 11402-1-APDownload protocol
IF protocol for EEF1A1 antibody 11402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Pathog Dis

Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.

Authors - Jia-Hui Deng
  • KD Validated
humanWB

Eur J Med Chem

Novel nannocystin A analogues as anticancer therapeutics: Synthesis, biological evaluations and structure-activity relationship studies.

Authors - Qiang Liu
humanWB

Nat Commun

RNA m6A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.

Authors - Xinyao Lin
humanWB

BMC Nephrol

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

Authors - Yang Lu
humanWB,IHC

Proteomics Clin Appl

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Authors - Bao Huimin H
humanWB,IHC,IF

J Pharm Biomed Anal

A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.

Authors - Alessandra Bosutti
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (08-25-2020)

This antibody successfully detects EEF1A1 in western blots. There was one specific and strong band at 50 kDa.

  • Applications: Western Blot
  • Cell Tissue Type: Heart