Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IF/ICC detected in | A431 cells, HaCaT cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83806-1-RR targets EGFR in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
| Calculated Molecular Weight | 134 kDa |
| Observed Molecular Weight | 145-165 kDa |
| GenBank Accession Number | NM_005228 |
| Gene Symbol | EGFR |
| Gene ID (NCBI) | 1956 |
| RRID | AB_3671393 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P00533 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for EGFR antibody 83806-1-RR | Download protocol |
| IF protocol for EGFR antibody 83806-1-RR | Download protocol |
| WB protocol for EGFR antibody 83806-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EGFR Recombinant monoclonal antibody (240603F10), catalog number 83806-1-RR, has 2 total citations. These appear in Ecotoxicology and Environmental Safety and International Immunopharmacology. The associated research fields include DEHP exposure and cardiac fibrosis risk via network toxicology, and the role of exosomal miRNA in macrophage polarization and oxidative stress.
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf DEHP exposure and cardiac fibrosis risk: An integrated network toxicology and molecular experimental investigation. | ||
Int Immunopharmacol The matrix of exosomes: Decoding the role of miRNA in macrophage polarization and oxidative stress |
Reviews
What customers say
Both reviews are 5-star ratings. One user reports the antibody works well for Western Blot on human cancer cell lines. Another user found it performed perfectly for Immunofluorescence at a 1:100 dilution, specifically marking a subtype of astrocytes in human brain cortical dysplasia tissue. Overall, reviewers highlight strong specificity and reliable performance across both WB and IF applications in human samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wen (Verified Customer) (04-02-2026) | It works well for human cancer cell lines
|
Reyes (Verified Customer) (09-24-2025) | EGFR (in green) worked perfectly marking a specific subtype of astrocytes in human brain cortical dysplasia tissue.
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