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EHHADH Polyclonal antibody

EHHADH Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26570-1-AP

Synonyms

ECHD, EHHADH, L PBE, LBFP, LBP, PBE, PBFE

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using EHHADH Polyclonal antibody (26570-1-AP)

    WB analysis using 26570-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using EHHADH Polyclonal antibody (26570-1-AP)

    WB analysis of SMMC-7721 using 26570-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of HepG2 cells using EHHADH Polyclonal antibody (26570-1-AP)

    WB analysis of HepG2 using 26570-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using EHHADH Polyclonal antibody (26570-1-AP)

    WB analysis of mouse liver using 26570-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26570-1-AP (EHHADH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EHHADH Polyclonal antibody (26570-1-AP)

    IHC staining of human liver cancer using 26570-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26570-1-AP (EHHADH Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using EHHADH Polyclonal antibody (26570-1-AP)

    IHC staining of human liver cancer using 26570-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26570-1-AP (EHHADH Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EHHADH Polyclonal antibody (26570-1-AP)

    IF Staining of HepG2 using 26570-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 26570-1-AP (EHHADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue, SMMC-7721 cells, HepG2 cells, mouse liver tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

The immunogen of 26570-1-AP is EHHADH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EHHADH fusion protein Ag24237
Full Name enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Calculated Molecular Weight 723 aa, 79 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC038948
Gene Symbol EHHADH
Gene ID (NCBI) 1962
RRIDAB_2880556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EHHADH antibody 26570-1-APDownload protocol
IHC protocol for EHHADH antibody 26570-1-APDownload protocol
IF protocol for EHHADH antibody 26570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
View Article
mouseWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang
View Article
mouseWB

Cell Rep

Feedback repression of PPARα signaling by Let-7 microRNA.

Authors - Tomoki Yagai
View Article
mouseWB

Int J Mol Sci

Ameliorative Effect of Ethanolic Echinacea purpurea against Hyperthyroidism-Induced Oxidative Stress via AMRK and PPAR Signal Pathway Using Transcriptomics and Network Pharmacology Analysis

Authors - Yingli Zhu
View Article