EIF2C1-Specific Polyclonal antibody

EIF2C1-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19690-1-AP

Synonyms

AGO1, Argonaute1, eIF 2C 1, EIF2C, eIF2C 1, EIF2C1, GERP95, hAgo1, Protein argonaute 1, Q99



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

19690-1-AP targets EIF2C1-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 2C, 1
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberNM_012199
Gene symbol EIF2C1
Gene ID (NCBI) 26523
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EIF2C1, also named as AGO1, belongs to the argonaute family and Ago subfamily. EIF2C1 is required for RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. EIF2C1 may also be required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). The antibody is specific to EIF2C1.

Protocols

Product Specific Protocols
WB protocol for EIF2C1-Specific antibody 19690-1-APDownload protocol
IHC protocol for EIF2C1-Specific antibody 19690-1-APDownload protocol
IF protocol for EIF2C1-Specific antibody 19690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Mol Neurosci

miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif.

Authors - Belinda J Goldie