EIF4E2 Polyclonal antibody

EIF4E2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12227-1-AP

Synonyms

4E LP, 4EHP, eIF 4E type 2, eIF4E like protein 4E LP, eIF4E type 2, EIF4E2, EIF4EL3, IF4e, mRNA cap binding protein 4EHP



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse kidney tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12227-1-AP targets EIF4E2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF4E2 fusion protein Ag2864
Full Name eukaryotic translation initiation factor 4E family member 2
Calculated molecular weight 245 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC021690
Gene symbol EIF4E2
Gene ID (NCBI) 9470
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF4E2 antibody 12227-1-APDownload protocol
IHC protocol for EIF4E2 antibody 12227-1-APDownload protocol
IP protocol for EIF4E2 antibody 12227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols