Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, mouse kidney tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12227-1-AP targets EIF4E2 in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2864 Product name: Recombinant human EIF4E2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-245 aa of BC021690 Sequence: MNNKFDALKDDDSGDHDQNEENSTQKDGEKEKTERDKNQSSSKRKAVVPGPAEHPLQYNYTFWYSRRTPGRPTSSQSYEQNIKQIGTFASVEQFWRFYSHMVRPGDLTGHSDFHLFKEGIKPMWEDDANKNGGKWIIRLRKGLASRCWENLILAMLGEQFMVGEEICGAVVSVRFQEDIISIWNKTASDQATTARIRDTLRRVLNLPPNTIMEYKTHTDSIKMPGRLGPQRLLFQNLWKPRLNVP Predict reactive species |
| Full Name | eukaryotic translation initiation factor 4E family member 2 |
| Calculated Molecular Weight | 245 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC021690 |
| Gene Symbol | EIF4E2 |
| Gene ID (NCBI) | 9470 |
| RRID | AB_10642945 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60573 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EIF4E2 antibody 12227-1-AP | Download protocol |
| IP protocol for EIF4E2 antibody 12227-1-AP | Download protocol |
| WB protocol for EIF4E2 antibody 12227-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-eIF4E2 antibody (Cat# 12227-1-AP) has 8 citations published in journals including Nature Communications, Cell Death & Disease, Nature Structural & Molecular Biology, Cell Reports, International Journal of Oncology, Scientific Reports, European Journal of Medical Research, and iScience. Research fields span translation regulation and ribosome quality control, RNA methylation, cancer biology (kidney, head and neck, glioma), DNA repair, myocardial ischemia-reperfusion injury, and SARS-CoV-2 viral protein interactions.
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Cell Death Dis α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation | ||
Nat Struct Mol Biol Isoform-resolved mRNA profiling of ribosome load defines interplay of HIF and mTOR dysregulation in kidney cancer | ||
Cell Rep eIF4E2 deficiency translationally unleashes WRN to sustain DNA repair-mediated chemoresistance. | ||
Int J Oncol piR‑164552 promotes malignant progression in head and neck squamous cell carcinoma via the RBM4/EIF4E2 signaling axis: Insights from multi‑omics and functional analyses. | ||
Sci Rep Comprehensive analysis of aberrant alternative splicing and RNA binding proteins regulators associated with myocardial ischemia reperfusion injury in mice. |
