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EIF4G1 Monoclonal antibody

EIF4G1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B10G8

Cat no : 67199-1-Ig

Synonyms

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4G1 Monoclonal antibody (67199-1-Ig)

    WB analysis using 67199-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using EIF4G1 Monoclonal antibody (67199-1-Ig)

    WB analysis of 4T1 using 67199-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF4G1 Monoclonal antibody (67199-1-Ig)

    IHC staining of mouse pancreas using 67199-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF4G1 Monoclonal antibody (67199-1-Ig)

    IHC staining of mouse pancreas using 67199-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF4G1 Monoclonal antibody (67199-1-Ig)

    IF Staining of HepG2 using 67199-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, 4T1 cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67199-1-Ig targets EIF4G1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF4G1 fusion protein Ag8357
Full Name eukaryotic translation initiation factor 4 gamma, 1
Calculated Molecular Weight 1600 aa, 176 kDa
Observed Molecular Weight 240-250 kDa
GenBank Accession NumberBC007788
Gene Symbol EIF4G1
Gene ID (NCBI) 1981
RRIDAB_2882492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Protocols

Product Specific Protocols
WB protocol for EIF4G1 antibody 67199-1-IgDownload protocol
IHC protocol for EIF4G1 antibody 67199-1-IgDownload protocol
IF protocol for EIF4G1 antibody 67199-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Antiviral Res

Proteomic and phosphoproteomic analysis of responses to enterovirus A71 infection reveals novel targets for antiviral and viral replication

Authors - Dandan Lin
View Article