EIF4G1 Monoclonal antibody, PBS Only

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  WB analysis using 67199-1-Ig (same clone as 67199-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67199-1-Ig (same clone as 67199-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67199-1-Ig (same clone as 67199-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4G1 antibody (67199-1-Ig, Clone: 2B10G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

EIF4G1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67199-1-PBS

Clone No.2B10G8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

2B10G8, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F

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Product Information

67199-1-PBS targets EIF4G1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8357

  Product name: Recombinant human EIF4G1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 296-645 aa of BC007788

  Sequence: IEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN

• Full Name: eukaryotic translation initiation factor 4 gamma, 1
• Calculated Molecular Weight: 1600 aa, 176 kDa
• Observed Molecular Weight: 240-250 kDa
• GenBank Accession Number: BC007788
• Gene Symbol: EIF4G1
• Gene ID (NCBI): 1981
• RRID: AB_2882492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q04637
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )