CoraLite®488-conjugated EIF4G1 Monoclonal antibody

EIF4G1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2B10G8

Cat no : CL488-67199

Synonyms

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67199-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67199 targets EIF4G1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF4G1 fusion protein Ag8357
Full Name eukaryotic translation initiation factor 4 gamma, 1
Calculated molecular weight 1600 aa, 176 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC007788
Gene symbol EIF4G1
Gene ID (NCBI) 1981
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm / 515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )

Protocols

Product Specific Protocols
IF protocol for CL488 EIF4G1 antibody CL488-67199Download protocol
Standard Protocols
Click here to view our Standard Protocols