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EMB Polyclonal antibody

EMB Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13946-1-AP

Synonyms

EMB, Embigin, embigin homolog (mouse)

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EMB Polyclonal antibody (13946-1-AP)

    WB analysis of HeLa using 13946-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EMB Polyclonal antibody (13946-1-AP)

    WB analysis of HeLa using 13946-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using EMB Polyclonal antibody (13946-1-AP)

    WB analysis of mouse skeletal muscle using 13946-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using EMB Polyclonal antibody (13946-1-AP)

    IHC staining of mouse testis using 13946-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using EMB Polyclonal antibody (13946-1-AP)

    IHC staining of mouse testis using 13946-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13946-1-AP targets EMB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EMB fusion protein Ag5031
Full Name embigin homolog (mouse)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC059398
Gene Symbol EMB
Gene ID (NCBI) 133418
RRIDAB_2877993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)

Protocols

Product Specific Protocols
WB protocol for EMB antibody 13946-1-APDownload protocol
IHC protocol for EMB antibody 13946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (10-04-2021)

calculated size 37 kd. This antibody recognized major bands at 65, 55 kd, and a very weak band ~45 kd. All of them were PNGaseF insensitive, suggesting they were not glycosylated form of this protein.

  • Applications: Western Blot