Emerin Polyclonal antibody

Emerin Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10351-1-AP

Synonyms

EDMD, EMD, Emerin, LEMD5, STA



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, MCF-7 cells, COLO 320 cells, A431 cells, Jurkat cells
Positive IP detected inmouse skeletal muscle tissue
Positive IF detected inHela cells, A431 cells, MCF-7 cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10351-1-AP targets Emerin in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Emerin fusion protein Ag0236
Full Name emerin
Calculated molecular weight 34 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC000738
Gene symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for Emerin antibody 10351-1-APDownload protocol
IF protocol for Emerin antibody 10351-1-APDownload protocol
FC protocol for Emerin antibody 10351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Targeting of LRRC59 to the Endoplasmic Reticulum and the Inner Nuclear Membrane.

Authors - Marina Blenski
humanWB,IF

Cells

Emerin Is Required for Proper Nucleus Reassembly after Mitosis: Implications for New Pathogenetic Mechanisms for Laminopathies Detected in EDMD1 Patients.

Authors - Magda Dubińska-Magiera
mouseWB

Cells

EDMD-Causing Emerin Mutant Myogenic Progenitors Exhibit Impaired Differentiation Using Similar Mechanisms.

Authors - Ashvin Iyer
mouseWB

BMC Cell Biol

Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression.

Authors - Elizabeth R Smith
humanWB,IF

Chromosoma

The effect of the lamin A and its mutants on nuclear structure, cell proliferation, protein stability, and mobility in embryonic cells.

Authors - Katarzyna Piekarowicz
humanWB, IF

Carcinogenesis

Autophagy-mediated degradation of nuclear envelope proteins during oncogene-induced senescence.

Authors - Christelle Lenain

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Joleen (Verified Customer) (06-12-2019)

Antibody recognizes clear band at the corrected expected molecular weight of Emerin.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
Emerin Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10351-1-AP)