Filter: AllWBIHCIF/ICC

WB analysis using 55234-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 55234-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 55234-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 55234-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55234-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55234-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 55234-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 55234-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 55234-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 55234-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 55234-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 55234-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 55234-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO3-specific antibody (55234-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

ENO3-specific Polyclonal antibody

ENO3-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55234-1-AP

Publications(5)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ENO3, MSE, GSD13, EC:4.2.1.11, Beta-enolase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells
Positive IHC detected in	mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 2 publications below

Product Information

55234-1-AP targets ENO3-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	enolase 3 (beta, muscle)
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	42-47 kDa
GenBank Accession Number	NM_001976
Gene Symbol	ENO3
Gene ID (NCBI)	2027
RRID	AB_10837363
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13929
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.

Protocols

Product Specific Protocols
WB protocol for ENO3-specific antibody 55234-1-AP	Download protocol
IHC protocol for ENO3-specific antibody 55234-1-AP	Download protocol
IF protocol for ENO3-specific antibody 55234-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
bovine	WB	J Proteomics Characterization of the liver proteome in dairy cows experiencing negative energy balance at early lactation. Authors - Turner H Swartz View Article
human	WB	Ann Transl Med ENO3 promoted the progression of NASH by negatively regulating ferroptosis via elevation of GPX4 expression and lipid accumulation. Authors - Di Lu KD Validated View Article
human	WB,IHC	Med Oncol ENO3 promotes colorectal cancer progression by enhancing cell glycolysis. Authors - Jingyu Chen KD Validated View Article
	WB	Reprod Domest Anim Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation. Authors - M D Xi View Article
Human	IHC	Technol Cancer Res Treat Predictive Models for Colon Adenocarcinoma Diagnosis, Prognosis, and Immune Microenvironment Based on 2 Hypoxia-Related Genes: KDM3A and ENO3 Authors - Chunli Kong View Article