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ENO3-specific Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 55234-1-AP

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Con: 114 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

ENO3-specific Antibody WB HeLa cells 55234-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

ENO3-specific Antibody IHC mouse skeletal muscle tissue 55234-1-AP

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ENO3-specific Antibody IHC mouse skeletal muscle tissue 55234-1-AP

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ENO3-specific Antibody IF HeLa cells 55234-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells
Positive IHC detected in:
mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:2000-1:10000
IHC : 1:250-1:1000
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
enolase 3 (beta, muscle)
Calculated molecular weight:
47 kDa
Observed molecular weight:
42-47 kDa
GenBank accession number:
NM_001976
Gene ID (NCBI):
2027
Gene symbol
ENO3
Synonyms
Beta enolase, ENO3, ENO3-specific, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

Background

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.


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