ENO3-specific Antibody 55234-1-AP | Proteintech

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ENO3-specific Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 55234-1-AP

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Freight/Packing

Con: 114 μg/150 μl

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20 μl 150ul

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Datasheet
Publications
Validation 20

Tested Applications

Applications:

ELISA, WB, IHC, IF
Application not tested? Please see our Guarantee

Images See all

55234-1-AP;Jurkat cells

Jurkat cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;multi-cells/tissue

WB result of 55234-1-AP

55234-1-AP;human skeletal muscle tissue

Immunohistochemical of paraffin-embedded human skeletal muscle using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 10x lens)

55234-1-AP;human skeletal muscle tissue

Immunohistochemical of paraffin-embedded human skeletal muscle using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 40x lens)

55234-1-AP;HeLa cells

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat

Positive WB detected in:

Jurkat cells, multi-cells/tissue

Positive IHC detected in:

human skeletal muscle tissue, human heart tissue, human skeletal muscle tissue

Positive IF detected in:

HeLa cells

Recommended dilution:

WB : 1:1000-1:4000

IHC : 1:20-1:200

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

Peptide

Full name:

enolase 3 (beta, muscle)

Calculated molecular weight:

47 kDa

Observed molecular weight:

42-47 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol

ENO3

Synonyms

Beta enolase, ENO3, ENO3-specific, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

Background

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.

WB Figures

55234-1-AP;HeLa cells

HeLa cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;HeLa cells

HeLa cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;HepG2 cells

HepG2 cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;HepG2 cells

HepG2 cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;Jurkat cells

Jurkat cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;Jurkat cells

Jurkat cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;mouse brain tissue

mouse brain tissue were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

55234-1-AP;mouse brain tissue

mouse brain tissue were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

55234-1-AP;multi-cells/tissue

WB result of 55234-1-AP

55234-1-AP;SH-SY5Y cells

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

55234-1-AP;SH-SY5Y cells

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55234-1-AP(ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

IHC Figures

55234-1-AP;human heart tissue

Immunohistochemical of paraffin-embedded human heart using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 10x lens)

55234-1-AP;human heart tissue

Immunohistochemical of paraffin-embedded human heart using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 40x lens)

55234-1-AP;human heart tissue

Immunohistochemical of paraffin-embedded human heart using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 10x lens)

55234-1-AP;human heart tissue

Immunohistochemical of paraffin-embedded human heart using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 40x lens)

55234-1-AP;human skeletal muscle tissue

Immunohistochemical of paraffin-embedded human skeletal muscle using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 10x lens)

55234-1-AP;human skeletal muscle tissue

Immunohistochemical of paraffin-embedded human skeletal muscle using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 40x lens)

55234-1-AP;human skeletal muscle tissue

Immunohistochemical of paraffin-embedded human skeletal muscle using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 10x lens)

55234-1-AP;human skeletal muscle tissue

Immunohistochemical of paraffin-embedded human skeletal muscle using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 (under 40x lens)

IF Figures

55234-1-AP;HeLa cells

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 55234-1-AP(ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)