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  • Featured Product
  • KD/KO Validated

ENO3-specific Polyclonal antibody

ENO3-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55234-1-AP

Synonyms

Beta enolase, ENO3, ENO3-specific, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENO3-specific Polyclonal antibody (55234-1-AP)

    WB analysis using 55234-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ENO3-specific Polyclonal antibody (55234-1-AP)

    WB analysis of Jurkat using 55234-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ENO3-specific Polyclonal antibody (55234-1-AP)

    WB analysis of SH-SY5Y using 55234-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ENO3-specific Polyclonal antibody (55234-1-AP)

    WB analysis of mouse brain using 55234-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ENO3-specific Polyclonal antibody (55234-1-AP)

    WB analysis of HeLa using 55234-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ENO3-specific Polyclonal antibody (55234-1-AP)

    WB analysis of HepG2 using 55234-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ENO3-specific Polyclonal antibody (55234-1-AP)

    IHC staining of mouse skeletal muscle using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ENO3-specific Polyclonal antibody (55234-1-AP)

    IHC staining of mouse skeletal muscle using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using ENO3-specific Polyclonal antibody (55234-1-AP)

    IHC staining of human heart using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ENO3-specific Polyclonal antibody (55234-1-AP)

    IHC staining of human heart using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ENO3-specific Polyclonal antibody (55234-1-AP)

    IHC staining of human skeletal muscle using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ENO3-specific Polyclonal antibody (55234-1-AP)

    IHC staining of human skeletal muscle using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENO3-specific Polyclonal antibody (55234-1-AP)

    IF Staining of HeLa using 55234-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells
Positive IHC detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55234-1-AP targets ENO3-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name enolase 3 (beta, muscle)
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 42-47 kDa
GenBank Accession NumberNM_001976
Gene Symbol ENO3
Gene ID (NCBI) 2027
RRIDAB_10837363
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.

Protocols

Product Specific Protocols
WB protocol for ENO3-specific antibody 55234-1-APDownload protocol
IHC protocol for ENO3-specific antibody 55234-1-APDownload protocol
IF protocol for ENO3-specific antibody 55234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB

J Proteomics

Characterization of the liver proteome in dairy cows experiencing negative energy balance at early lactation.

Authors - Turner H Swartz
View Article
humanWB

Ann Transl Med

ENO3 promoted the progression of NASH by negatively regulating ferroptosis via elevation of GPX4 expression and lipid accumulation.

Authors - Di Lu
  • KD Validated
View Article
humanWB,IHC

Med Oncol

ENO3 promotes colorectal cancer progression by enhancing cell glycolysis.

Authors - Jingyu Chen
  • KD Validated
View Article
WB

Reprod Domest Anim

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.

Authors - M D Xi
View Article