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ENO3-specific Polyclonal antibody

ENO3-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55234-1-AP

Synonyms

Beta enolase, ENO3, ENO3-specific, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase

Validation Data Gallery

  • WB analysis using 55234-1-AP

    WB analysis using 55234-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55234-1-AP (ENO3-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 55234-1-AP

    IHC staining of mouse skeletal muscle using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 55234-1-AP

    IHC staining of mouse skeletal muscle using 55234-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55234-1-AP (ENO3-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 55234-1-AP

    IF Staining of HeLa using 55234-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55234-1-AP (ENO3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells
Positive IHC detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

55234-1-AP targets ENO3-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name enolase 3 (beta, muscle)
Calculated molecular weight 47 kDa
Observed molecular weight 42-47 kDa
GenBank accession numberNM_001976
Gene symbol ENO3
Gene ID (NCBI) 2027
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13). This antibody is specific to ENO3. It has no cross reaction to ENO1 and ENO2.

Protocols

Product Specific Protocols
WB protocol for ENO3-specific antibody 55234-1-APDownload protocol
IHC protocol for ENO3-specific antibody 55234-1-APDownload protocol
IF protocol for ENO3-specific antibody 55234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Reprod Domest Anim

Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.

Authors - M D Xi
View Article