Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human tonsillitis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84235-4-RR targets CD39/ENTPD1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant Protein Predict reactive species |
| Full Name | ectonucleoside triphosphate diphosphohydrolase 1 |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC047664 |
| Gene Symbol | CD39 |
| Gene ID (NCBI) | 953 |
| RRID | AB_3671788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P49961 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms(58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD39/ENTPD1 antibody 84235-4-RR | Download protocol |
| WB protocol for CD39/ENTPD1 antibody 84235-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD39/ENTPD1 Recombinant monoclonal antibody (clone 241292A1), catalog number 84235-4-RR, has 1 total citation. The cited publication appeared in Neurochemistry International (2025) and investigated the role of CD39 and CD73 upregulation in microglia following subacute cervical spinal cord injury, linking increased spinal adenosine to sustained ectonucleotidase expression. The research field encompasses neurochemistry, spinal cord injury, and purinergic signaling in glial cells.
| Species | Application | Title |
|---|---|---|
Neurochem Int Increased spinal adenosine after subacute cervical injury correlates with sustained upregulation of CD39 and CD73 in microglia |
