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  • Featured Product
  • KD/KO Validated

EPHA2 Monoclonal antibody

EPHA2 Monoclonal Antibody for FC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1A9C3

Cat no : 66736-1-Ig

Synonyms

ECK, EPH receptor A2, EPHA2, Ephrin type A receptor 2, Epithelial cell kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPHA2 Monoclonal antibody (66736-1-Ig)

    WB analysis using 66736-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using EPHA2 Monoclonal antibody (66736-1-Ig)

    FC experiment of HeLa using 66736-1-Ig

    1X10^6 Hela cells were stained with 0.20ug EPHA2 antibody (66736-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, BxPC-3 cells, MCF-7 cells, HepG2 cells
Positive FC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66736-1-Ig targets EPHA2 in WB, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPHA2 fusion protein Ag22566
Full Name EPH receptor A2
Calculated Molecular Weight 976 aa, 108 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC037166
Gene Symbol EPHA2
Gene ID (NCBI) 1969
RRIDAB_2882086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EPHA2 antibody 66736-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther Nucleic Acids

EFNA4 promotes cell proliferation and tumor metastasis in hepatocellular carcinoma through a PIK3R2/GSK3β/β-catenin positive feedback loop.

Authors - Junhao Lin
View Article
humanWB

Int J Mol Sci

Role of Nuclear Claudin-4 in Renal Cell Carcinoma.

Authors - Takuya Owari
View Article
human,mouseWB

J Oncol

EphA2 Promotes the Development of Cervical Cancer through the CXCL11/PD-L1 Pathway

Authors - Xinyue Zhao
  • KD Validated
View Article
mouseWB

J Alzheimers Dis

Metabolic Reprogramming of Microglia Enhances Proinflammatory Cytokine Release through EphA2/p38 MAPK Pathway in Alzheimer's Disease

Authors - Xiaowei Ma
  • KD Validated
View Article
humanWB

Chem Biol Interact

2-Methoxy-5((3,4,5-trimethosyphenyl)seleninyl) phenol (SQ) inhibits cancer cell metastasis behavior of TNBC via suppressing EMT and VEGF.

Authors - Dayong Zheng
View Article
humanWB

Arch Biochem Biophys

EphA2 inhibits SRA01/04 cells apoptosis by suppressing autophagy via activating PI3K/Akt/mTOR pathway.

Authors - Jing Han
  • KD Validated
View Article