Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11257-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11257-1-AP
WB result of ERK1/2 antibody (11257-1-AP, 1:1000) with HeLa and HEK293 cells.
WB analysis using 11257-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11257-1-AP (ERK1/2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11257-1-AP
WB result of ERK1/2 antibody (11257-1-AP, 1:2000) with si-Control and si-ERK1/2 transfected HeLa cells.
IP experiment of HEK-293 using 11257-1-AP
IP result of anti-ERK1/2 (IP:11257-1-AP, 4ug; Detection:11257-1-AP 1:3000) with HEK-293 cells lysate 1440 ug.
IHC staining of human colon cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11257-1-AP (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 11257-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 11257-1-AP (ERK1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HEK-293 using 11257-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ERK1/2 antibody (11257-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of HeLa using 11257-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ERK1/2 (11257-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ERK1/2 Polyclonal antibody

ERK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 11257-1-AP

Publications(461)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ERK, ERK1, ERK2, EC:2.7.11.24, ERK-1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (461)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	PC-12 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse lung tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 447 publications below
IHC	See 10 publications below
IF	See 14 publications below
CoIP	See 2 publications below

Product Information

11257-1-AP targets ERK1/2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, chicken, zebrafish, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERK1/2 fusion protein Ag1759 Predict reactive species
Full Name	mitogen-activated protein kinase 3
Calculated Molecular Weight	43 kDa
Observed Molecular Weight	38-44 kDa
GenBank Accession Number	BC013992
Gene Symbol	ERK1
Gene ID (NCBI)	5595
RRID	AB_2139822
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P27361
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
WB protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IHC protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IF protocol for ERK1/2 antibody 11257-1-AP	Download protocol
IP protocol for ERK1/2 antibody 11257-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer Authors - Yurou Chen View Article
mouse	WB	Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure Authors - Lingjun Tong View Article
human	WB	Redox Biol Twin defect-rich Pt ultrathin nanowire nanozymes alleviate inflammatory skin diseases by scavenging reactive oxygen species Authors - He Zhao View Article
	WB	Cancer Res Osimertinib covalently binds to CD34 and eliminates myeloid leukemia stem/progenitor cells Authors - Li Xia View Article
mouse	WB	EMBO Mol Med Luteolin detoxifies DEHP and prevents liver injury by degrading Uroc1 protein in mice Authors - Huiting Wang View Article
mouse	WB	ACS Appl Mater Interfaces Near-Infrared Light-Responsive Nanoinhibitors for Tumor Suppression through Targeting and Regulating Anion Channels. Authors - Chaoqun Li View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Charlotte (Verified Customer) (08-15-2024)	The company suggests us this ERK ab as the other one we bought from them was not working. I overlaid ERK with pERK and it matches nicely. Satisfied of this antibody. Done in milk. Applications: Western Blot Primary Antibody Dilution: 1/5000 Cell Tissue Type: HT29 ERK1/2 Antibody Western Blot validation (1/5000 dilution) in HT29 (Cat no:11257-1-AP)
FH XB (Verified Customer) (10-29-2020)	A reliable ab to detect total ERK1/2 by Western blotting. Applications: Western Blot Primary Antibody Dilution: 1:3000 Cell Tissue Type: mouse brain ERK1/2 Antibody Western Blot validation (1:3000 dilution) in mouse brain (Cat no:11257-1-AP)