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ERK1/2 Antibody

ERK1/2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11257-1-AP

Synonyms

ERK, ERK 1, ERK 1/2, ERK1, ERK1/2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, A375 cells, A549 cells, Jurkat cells, mouse lung tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells, MCF-7 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11257-1-AP targets ERK1/2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERK1/2 fusion protein Ag1759
Full Name mitogen-activated protein kinase 3
Calculated molecular weight 43 kDa
Observed molecular weight 38-44 kDa
GenBank accession numberBC013992
Gene symbol MAPK3
Gene ID (NCBI) 5595
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Protocols

Product Specific Protocols
WB protocol for ERK1/2 antibody 11257-1-APDownload protocol
IHC protocol for ERK1/2 antibody 11257-1-APDownload protocol
IF protocol for ERK1/2 antibody 11257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Chin J Nat Med

Sodium tanshinone IIA sulfonate prevents radiation-induced damage in primary rat cardiac fibroblasts.

Authors - Dan Zhou
mouseWB

Cell Death Dis

Hfm1 participates in Golgi-associated spindle assembly and division in mouse oocyte meiosis.

Authors - Huiyuan Wang
WB

Mol Med Rep

Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway.

Authors - Xiaoxia Li
humanWB

J Gene Med

MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.

Authors - Wei-Yan Qin
humanWB

Int J Radiat Biol

PMID: 23682582

Authors - Xiao-Fang Dai
humanWB

Onco Targets Ther

PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation.

Authors - Jian-Min Lv