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- KD/KO Validated
ERK1/2 Polyclonal antibody
ERK1/2 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11257-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, A375 cells, A549 cells, Jurkat cells, mouse lung tissue |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, human prostate cancer tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A375 cells, MCF-7 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 23 publications below |
| IHC | See 1 publications below |
Product Information
11257-1-AP targets ERK1/2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ERK1/2 fusion protein Ag1759 |
| Full Name | mitogen-activated protein kinase 3 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 38-44 kDa |
| GenBank accession number | BC013992 |
| Gene symbol | MAPK3 |
| Gene ID (NCBI) | 5595 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
| IHC protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
| IF protocol for ERK1/2 antibody 11257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Chin J Nat Med Sodium tanshinone IIA sulfonate prevents radiation-induced damage in primary rat cardiac fibroblasts. | ||
Cell Death Dis Hfm1 participates in Golgi-associated spindle assembly and division in mouse oocyte meiosis. | ||
Mol Med Rep Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway. | ||
Onco Targets Ther PPP5C promotes cell proliferation and survival in human prostate cancer by regulating of the JNK and ERK1/2 phosphorylation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH XB (Verified Customer) (10-29-2020) | A reliable ab to detect total ERK1/2 by Western blotting.
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