|Positive WB detected in||mouse brain tissue, HeLa cells, HepG2 cells, mouse skin tissue, rat skeletal muscle tissue, NIH/3T3 cells, A431 cells, HEK-293 cells, rat skin tissue|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
51068-1-AP targets ERK1/2 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||mitogen-activated protein kinase 1|
|Calculated molecular weight||360 aa, 41 kDa|
|Observed molecular weight||38-44 kDa|
|GenBank accession number||BC017832|
|Gene ID (NCBI)||5594|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
|Product Specific Protocols|
|WB protocol for ERK1/2 antibody 51068-1-AP||Download protocol|
|IHC protocol for ERK1/2 antibody 51068-1-AP||Download protocol|
|IF protocol for ERK1/2 antibody 51068-1-AP||Download protocol|
|FC protocol for ERK1/2 antibody 51068-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Med Rep
Formononetin inhibits colon carcinoma cell growth and invasion by microRNA‑149‑mediated EphB3 downregulation and inhibition of PI3K/AKT and STAT3 signaling pathways.
A systems biology-based classifier for hepatocellular carcinoma diagnosis.
Suhuang Antitussive Capsules-Ameliorative Effects on LPS-Induced Sputum Obstruction in Mice Through Promoting HGF Secretion.
Siglec-F is induced by GM-CSF and enhances IL-4-induced expression of arginase-1 in mouse macrophages.
Urokinase-type plasminogen activator receptor signaling is critical in nasopharyngeal carcinoma cell growth and metastasis.
The PI3Kα inhibitor DFX24 suppresses tumor growth and metastasis in non-small cell lung cancer via ERK inhibition and EPHB6 reactivation.