Tested Applications
| Positive WB detected in | BGC-823 cells, HeLa cells, mouse liver tissue |
| Positive IHC detected in | human liver tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
27786-1-AP targets ETHE1 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26597 Product name: Recombinant human ETHE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 8-132 aa of BC008250 Sequence: VARRQLSQRGGSGAPILLRQMFEPVSCTFTYLLGDRESREAVLIDPVLETAPRDAQLIKELGLRLLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADLHIEDGDSIRFGRFALETRAS Predict reactive species |
| Full Name | ethylmalonic encephalopathy 1 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC008250 |
| Gene Symbol | ETHE1 |
| Gene ID (NCBI) | 23474 |
| RRID | AB_2880971 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95571 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ETHE1, a gene encoding a b-lactamase-like, iron-coordinating metalloprotein, is mutated in ethylmalonic encephalopathy.In humans, ETHE1 gene is proved to encode the SDO in mitochondria and its mutations lead to a fatal autosomal recessive mitochondrial disease: ethylmalonic encephalopathy.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ETHE1 antibody 27786-1-AP | Download protocol |
| WB protocol for ETHE1 antibody 27786-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma | ||
Int J Biol Sci Metformin Alleviates Liver Metabolic Dysfunction in Polycystic Ovary Syndrome by Activating the Ethe1/Keap1/PINK1 Pathway |
