FIP1L1 Polyclonal antibody

FIP1L1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12276-1-AP

Synonyms

Factor interacting with PAP, FIP1, FIP1 like 1 (S. cerevisiae), FIP1 like 1 protein, FIP1L1, FIP1L1 PDGFRalpha, hFip1, Rhe



Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12276-1-AP targets FIP1L1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FIP1L1 fusion protein Ag2921
Full Name FIP1 like 1 (S. cerevisiae)
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC017724
Gene Symbol FIP1L1
Gene ID (NCBI) 81608
RRIDAB_2231572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FIP1L1 antibody 12276-1-APDownload protocol
IHC protocol for FIP1L1 antibody 12276-1-APDownload protocol
IF protocol for FIP1L1 antibody 12276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols