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  • Featured Product
  • KD/KO Validated

FTO Polyclonal antibody

FTO Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Cat no : 27226-1-AP

Synonyms

FTO, KIAA1752

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FTO Polyclonal antibody (27226-1-AP)

    WB analysis using 27226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FTO Polyclonal antibody (27226-1-AP)

    WB analysis using 27226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27226-1-AP (FTO antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using FTO Polyclonal antibody (27226-1-AP)

    IP experiment of mouse brain using 27226-1-AP

    IP result of anti-FTO (IP:27226-1-AP, 4ug; Detection:27226-1-AP 1:1000) with mouse brain tissue lysate 2080 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of mouse testis using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of mouse testis using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human breast cancer using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human breast cancer using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human stomach cancer using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human stomach cancer using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human colon using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human colon using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human liver cancer using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of human liver cancer using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of mouse brain using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FTO Polyclonal antibody (27226-1-AP)

    IHC staining of mouse brain using 27226-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27226-1-AP (FTO antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using FTO Polyclonal antibody (27226-1-AP)

    IF Staining of human lymphoma using 27226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human lymphoma tissue using FTO Polyclonal antibody (27226-1-AP)

    IF Staining of human lymphoma using 27226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IF Staining of human liver cancer using 27226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using FTO Polyclonal antibody (27226-1-AP)

    IF Staining of human liver cancer using 27226-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTO antibody (27226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using FTO Polyclonal antibody (27226-1-AP)

    FC experiment of SH-SY5Y using 27226-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human FTO (27226-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, Caco-2 cells, HEK-293 cells, HeLa cells, HEK-293T cells, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, human liver cancer tissue, mouse brain tissue, human colon tissue, human stomach cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman lymphoma tissue, human liver cancer tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27226-1-AP targets FTO in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTO fusion protein Ag26095
Full Name fat mass and obesity associated
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_001080432
Gene Symbol FTO
Gene ID (NCBI) 79068
RRIDAB_2880809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fat mass and obesity-associated protein (FTO) has efficient oxidative demethylation activity targeting the abundant N6-methyladenosine (m6A) residues in RNA in vitro. Variants in the FTO (fat mass and obesity associated) gene are associated with increased body mass index in humans.

Protocols

Product Specific Protocols
WB protocol for FTO antibody 27226-1-APDownload protocol
IHC protocol for FTO antibody 27226-1-APDownload protocol
IF protocol for FTO antibody 27226-1-APDownload protocol
IP protocol for FTO antibody 27226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

A druggable copper-signalling pathway that drives inflammation

Authors - Stéphanie Solier
View Article
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
  • KO Validated
View Article
mouseWB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
View Article
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
View Article
humanWB

Nat Commun

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

Authors - Shujie Chen
View Article
humanWB

Dev Cell

PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.

Authors - Deguan Lv
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Katherine (Verified Customer) (09-25-2023)

Quality was good.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
FH

S (Verified Customer) (03-20-2023)

Very Good

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: AC16
FTO Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:27226-1-AP)
FH

SU (Verified Customer) (03-22-2022)

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)
FH

P. (Verified Customer) (05-15-2021)

Excellent antibody!

  • Applications: Western Blot
  • Cell Tissue Type: H9C2
FTO Antibody Western Blot validation ( dilution) in H9C2 (Cat no:27226-1-AP)