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  • KD/KO Validated

FTO Polyclonal antibody

FTO Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 27226-1-AP

Synonyms

FTO, KIAA1752



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, mouse brain tissue, HeLa cells, Caco-2 cells
Positive IHC detected inmouse brain tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27226-1-AP is FTO Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FTO fusion protein Ag26095
Full Name fat mass and obesity associated
Calculated molecular weight 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberNM_001080432
Gene symbol FTO
Gene ID (NCBI) 79068
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FTO antibody 27226-1-APDownload protocol
IHC protocol for FTO antibody 27226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Epigenomics

Alteration of N6-methyladenosine epitranscriptome profile in unilateral ureteral obstructive nephropathy.

Authors - Xueyan Li
mouseWB

Animals (Basel)

Resveratrol Attenuates Aflatoxin B1-Induced ROS Formation and Increase of m6A RNA Methylation.

Authors - Jiamin Wu
humanWB

Cell Death Dis

METTL14-regulated PI3K/Akt signaling pathway via PTEN affects HDAC5-mediated epithelial-mesenchymal transition of renal tubular cells in diabetic kidney disease.

Authors - Zhaoxia Xu
humanWB,IHC

Pathol Res Pract

FTO accelerates ovarian cancer cell growth by promoting proliferation, inhibiting apoptosis, and activating autophagy.

Authors - Lin Zhao
  • KD Validated
mouseWB,IHC

Arch Gynecol Obstet

Decreased expression of m6A demethylase FTO in ovarian aging.

Authors - Xiaoyan Sun
humanWB,IHC

Exp Cell Res

FTO promotes cell proliferation and migration in esophageal squamous cell carcinoma through up-regulation of MMP13.

Authors - Shenxiang Liu
  • KD Validated