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GAB1 Polyclonal antibody

GAB1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 26200-1-AP

Synonyms

GAB1, GRB2 associated binder 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GAB1 Polyclonal antibody (26200-1-AP)

    WB analysis using 26200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26200-1-AP (GAB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GAB1 Polyclonal antibody (26200-1-AP)

    WB analysis of HEK-293 using 26200-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26200-1-AP (GAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26200-1-AP is GAB1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAB1 fusion protein Ag24395
Full Name GRB2-associated binding protein 1
Calculated Molecular Weight 724 aa, 80 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC064848
Gene Symbol GAB1
Gene ID (NCBI) 2549
RRIDAB_2918101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GAB1 antibody 26200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article
humanWB,IF

Toxicol Appl Pharmacol

Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways.

Authors - Nai-Jie Fu
View Article