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  • KD/KO Validated

GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18723-1-AP

Synonyms

FLC3B, GABARAP, MM46



Tested Applications

Positive WB detected inhuman placenta tissue, A549 cells, HeLa cells, human heart tissue, mouse brain tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue
Positive IF detected inHepG2 cells, Starvation treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18723-1-AP targets GABARAP in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name GABA(A) receptor-associated protein
Calculated molecular weight 14 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC106748
Gene symbol GABARAP
Gene ID (NCBI) 11337
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.

Protocols

Product Specific Protocols
WB protocol for GABARAP antibody 18723-1-APDownload protocol
IF protocol for GABARAP antibody 18723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,monkeyCoIP,WB

Curr Biol

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.

Authors - Qingzhou Chen
  • KO Validated
mouseCoIP

Cell Death Dis

Protrudin modulates seizure activity through GABAA receptor regulation.

Authors - Xi Lu
humanWB

Cells

The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles.

Authors - Julia L Sanwald
mouseWB

Eur J Cell Biol

The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.

Authors - Dorthe Labonté
humanWB

Sci Rep

Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.

Authors - Alexandra Boeske
humanWB

Cell Commun Signal

Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components.

Authors - Paula Lindner