|Positive WB detected in||human placenta tissue, A549 cells, HeLa cells, human heart tissue, mouse brain tissue, mouse liver tissue, mouse pancreas tissue, mouse skeletal muscle tissue|
|Positive IF detected in||HepG2 cells, Starvation treated HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18723-1-AP targets GABARAP in WB, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||GABA(A) receptor-associated protein|
|Calculated molecular weight||14 kDa|
|Observed molecular weight||14 kDa|
|GenBank accession number||BC106748|
|Gene ID (NCBI)||11337|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.
Cell Death Dis
Protrudin modulates seizure activity through GABAA receptor regulation.
The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles.
Eur J Cell Biol
The kinesin KIF21B participates in the cell surface delivery of γ2 subunit-containing GABAA receptors.
Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization.
Cell Commun Signal
Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components.