Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, mouse brain tissue, human placenta tissue, mouse pancreas tissue, human heart tissue, mouse skeletal muscle tissue, HuH-7 cells, SH-SY5Y cells, RAW 264.7 cells, mouse liver tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Starvation treated HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18723-1-AP targets GABARAP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | GABA(A) receptor-associated protein |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14-18 kDa |
| GenBank Accession Number | BC106748 |
| Gene Symbol | GABARAP |
| Gene ID (NCBI) | 11337 |
| RRID | AB_10603365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95166 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), was initially identified as GABAA receptor binding protein and has shown to be implicated in intracellular trafficking of GABAA receptors. Through interacting with a broad rang of proteins, GABARAP has been demonstrated as a multifunctional protein implicated in intracellular vesicle trafficking, apoptosis, and autophagy. The protein level of lipidated form of GABARAP (GABARAP-II) is upregulated during the autophagic process, which makes it as an autophagic marker. This antibody detected endogenous GABARAP protein in lysates from various tissues. It may cross-react with GABARAPL1 due to the high identity of amino acid sequence.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GABARAP antibody 18723-1-AP | Download protocol |
| IF protocol for GABARAP antibody 18723-1-AP | Download protocol |
| IHC protocol for GABARAP antibody 18723-1-AP | Download protocol |
| IP protocol for GABARAP antibody 18723-1-AP | Download protocol |
| WB protocol for GABARAP antibody 18723-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GABARAP Polyclonal antibody (Cat# 18723-1-AP) has 38 citations published in journals including Nature, Autophagy, Nature Communications, Molecular Cell, EMBO Journal, Cell Reports, and Current Biology. Associated research fields span selective autophagy, ER-phagy, STING innate immune signaling, neurodegeneration (ALS/FTD), cancer, viral infection, lysosomal quality control, GABA receptor trafficking, pulmonary fibrosis, and cell death.
| Species | Application | Title |
|---|---|---|
Autophagy ATG4B antagonizes antiviral immunity by GABARAP-directed autophagic degradation of TBK1
| ||
Autophagy A fetus with severe developmental defects caused by dominant-negative and hypomorphic ATG7 alleles. | ||
Autophagy ATF3 -activated accelerating effect of LINC00941/lncIAPF on fibroblast-to-myofibroblast differentiation by blocking autophagy depending on ELAVL1/HuR in pulmonary fibrosis
| ||
Autophagy Advancing targeted protein degradation: pLIRTAC's role in glioma and CAR-T cell therapy.
| ||
Reviews
What customers say
All three reviews are for Western Blot. Two from the same user (rated 4/5) report the antibody works very well with 5% milk blocking at a 1/3000 dilution on HepG2 cells. A third reviewer (rated 3/5) used HeLa cell protein extract at 1:1000 dilution. Overall, users find this antibody performs reliably in WB, particularly when paired with milk-based blocking buffers.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hala (Verified Customer) (05-09-2023) | works very good in milk 5%
 |
Hala (Verified Customer) (04-28-2023) | works very good with milk blocking
|
Anita (Verified Customer) (12-22-2020) | HeLa cell protein extract (5 micrograms)
 |
