Galectin-3 Antibody 14979-1-AP

Tested Applications

Applications:

ELISA, IF, IHC, IP, WB
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WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells.

Immunohistochemical of paraffin-embedded human thyroid cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human thyroid cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 40x lens)

IP Result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug.

MCF-7 cells were subjected to SDS PAGE followed by western blot with 14979-1-AP( Galectin-3 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours

Immunofluorescent analysis of MCF-7 cells using 14979-1-AP(Galectin-3 antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat

Positive WB detected in:

MCF-7 cells, HeLa cells

Positive IP detected in:

MCF-7 cells

Positive IHC detected in:

human thyroid cancer tissue, human colon cancer tissue, human thyroid cancer tissue

Positive IF detected in:

MCF-7 cells

Recommended dilution:

WB : 1:500-1:3000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:20-1:200

IF : 1:10-1:100

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

Galectin-3 fusion protein ag6891

Full name:

lectin, galactoside-binding, soluble, 3

Calculated molecular weight:

26 kDa

Observed molecular weight:

31 kDa

GenBank accession number:

BC001120

Gene ID (NCBI):

3958

Gene symbol

LGALS3

Synonyms

35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2

Background

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3 contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers. Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).

Showing 1- [3] of [3] Publications

Author:		Eadon Michael T MT
Pubmed ID:		23922129
Journal:		Physiol Genomics
Title:		Cell cycle arrest in a model of colistin nephrotoxicity.
Application:

Species:

Author:		Ruo-Nan Zhang
Pubmed ID:		24951754
Journal:		Am J Physiol Heart Circ Physiol
Title:		Tongxinluo inhibits vascular inflammation and neointimal hyperplasia through blocking positive feedback loop between miR-155 and TNF-α
Application:		IHC

Species:		mouse

Author:		Jingni He
Pubmed ID:		28431936
Journal:		J Am Soc Hypertens
Title:		Galectin-3 mediates the pulmonary arterial hypertension-induced right ventricular remodeling through interacting with NADPH oxidase 4.
Application:		WB,IHC,IF

Species:		rat

WB Figures

HeLa cells were subjected to SDS PAGE followed by western blot with 14979-1-AP(Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells.

HeLa cells were subjected to SDS PAGE followed by western blot with 14979-1-AP( Galectin-3 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours

human heart tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP(Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 14979-1-AP(Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

MCF-7 cells were subjected to SDS PAGE followed by western blot with 14979-1-AP( Galectin-3 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14979-1-AP( Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

rat colon tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP( Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

IHC Figures

Immunohistochemical of paraffin-embedded human colon cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human colon cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemical of paraffin-embedded human colon cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human colon cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP( Galectin-3 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP( Galectin-3 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemical of paraffin-embedded human thyroid cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human thyroid cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 40x lens)

IP Figures

IP Result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug.

IF Figures

Immunofluorescent analysis of MCF-7 cells using 14979-1-AP(Galectin-3 antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Galectin-3 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 14979-1-AP

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Tested Applications

Product Information

Immunogen Information

Background

Showing 1- [3] of [3] Publications

WB Figures

IHC Figures

IP Figures

IF Figures

Galectin-3 Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 14979-1-AP

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Related Primary Antibodies

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Tested Applications

Product Information

Immunogen Information

Background