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Galectin-3 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 14979-1-AP

Featured Product KD/KO validated

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Galectin-3 Antibody WB HeLa cells 14979-1-AP

WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells.

Galectin-3 Antibody IHC human thyroid cancer tissue 14979-1-AP

Immunohistochemical of paraffin-embedded human thyroid cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 10x lens)

Galectin-3 Antibody IHC human thyroid cancer tissue 14979-1-AP

Immunohistochemical of paraffin-embedded human thyroid cancer using 14979-1-AP(Galectin-3 antibody) at dilution of 1:50 (under 40x lens)

Galectin-3 Antibody IP MCF-7 cells 14979-1-AP

IP Result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug.

Galectin-3 Antibody WB MCF-7 cells 14979-1-AP

MCF-7 cells were subjected to SDS PAGE followed by western blot with 14979-1-AP( Galectin-3 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours

Galectin-3 Antibody IF MCF-7 cells 14979-1-AP

Immunofluorescent analysis of MCF-7 cells using 14979-1-AP(Galectin-3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
MCF-7 cells, HeLa cells, human heart tissue, NIH/3T3 cells, rat colon tissue
Positive IP detected in:
MCF-7 cells
Positive IHC detected in:
human thyroid cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Galectin-3 fusion protein Ag6891
Full name:
lectin, galactoside-binding, soluble, 3
Calculated molecular weight:
26 kDa
Observed molecular weight:
31 kDa
GenBank accession number:
BC001120
Gene ID (NCBI):
3958
Gene symbol
LGALS3
Synonyms
35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2

Background

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).


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