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Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, HepG2 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue, human placenta tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83324-1-RR targets GANAB in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag30417 Product name: Recombinant human GANAB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-488 aa of BC017435 Sequence: GGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR Predict reactive species |
| Full Name | glucosidase, alpha; neutral AB |
| Calculated Molecular Weight | 944aa,107 kDa; 488aa,55 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC017435 |
| Gene Symbol | GANAB |
| Gene ID (NCBI) | 23193 |
| RRID | AB_3670990 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14697 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GANAB antibody 83324-1-RR | Download protocol |
| IF protocol for GANAB antibody 83324-1-RR | Download protocol |
| IHC protocol for GANAB antibody 83324-1-RR | Download protocol |
| WB protocol for GANAB antibody 83324-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
