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  • Featured Product
  • KD/KO Validated

KGA/GAC Polyclonal antibody

KGA/GAC Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Cat no : 12855-1-AP

Synonyms

AAD20, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAC, KIAA0838, L glutamine amidohydrolase

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis of HEK-293 using 12855-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of various lysates using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis of HEK-293 using 12855-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis of mouse brain using 12855-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis of mouse kidney using 12855-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using KGA/GAC Polyclonal antibody (12855-1-AP)

    WB analysis of COLO 320 using 12855-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IP experiment of mouse brain using 12855-1-AP

    IP result of anti-KGA/GAC (IP:12855-1-AP, 4ug; Detection:12855-1-AP 1:5000) with mouse brain tissue lysate 2400 ug.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IP experiment of mouse brain using 12855-1-AP

    IP result of anti-KGA/GAC (IP:12855-1-AP, 4ug; Detection:12855-1-AP 1:5000) with mouse brain tissue lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human gliomas using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human gliomas using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human kidney using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human kidney using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human brain using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human brain using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human gliomas using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using KGA/GAC Polyclonal antibody (12855-1-AP)

    IHC staining of human gliomas using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using KGA/GAC Polyclonal antibody (12855-1-AP)

    IF Staining of HEK-293 using 12855-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using KGA/GAC Polyclonal antibody (12855-1-AP)

    IF Staining of L02 using 12855-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using KGA/GAC antibody (12855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using KGA/GAC Polyclonal antibody (12855-1-AP)

    FC experiment of HeLa using 12855-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KGA/GAC (12855-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, mouse kidney tissue, HEK-293 cells, rat liver tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, L02 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12855-1-AP targets KGA/GAC in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, cat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KGA/GAC fusion protein Ag3505
Full Name glutaminase
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight58 kDa, 65 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_2110381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

Protocols

Product Specific Protocols
WB protocol for KGA/GAC antibody 12855-1-APDownload protocol
IHC protocol for KGA/GAC antibody 12855-1-APDownload protocol
IF protocol for KGA/GAC antibody 12855-1-APDownload protocol
IP protocol for KGA/GAC antibody 12855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Authors - Chioniso P Masamha
View Article
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou
View Article
mouseWB

Cell

Systemic elevation of PTEN induces a tumor-suppressive metabolic state.

Authors - Garcia-Cao Isabel I
View Article
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen
View Article
mouseWB

Nat Metab

Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis

Authors - Grace Park
View Article
ratWB

Brain Behav Immun

Modulation of immune function by glutamatergic neurons in the cerebellar interposed nucleus via hypothalamic and sympathetic pathways.

Authors - Fen-Fen Xu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Kishor (Verified Customer) (01-13-2020)

Anti body works for human and mouse proteins. 1:1000 dilution in BSA+TBST is good.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue
KGA/GAC Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:12855-1-AP)