GOLGA2/GM130 Monoclonal antibody

GOLGA2/GM130 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2A4F11

Cat no : 66662-1-Ig

Synonyms

GM130, GM130 autoantigen, GOLGA2, Golgin 95, Golgin subfamily A member 2



Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, HeLa cells, K-562 cells, PC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

66662-1-Ig targets GOLGA2/GM130 in WB,ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight 111 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC014188
Gene symbol GOLGA2
Gene ID (NCBI) 2801
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protocols

Product Specific Protocols
WB protocol for GOLGA2/GM130 antibody 66662-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols