GMDS Polyclonal antibody

GMDS Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15442-1-AP

Synonyms

GDP D mannose dehydratase, GDP mannose 4,6 dehydratase, GMD, GMDS, SDR3E1



Tested Applications

Positive WB detected inmouse pancreas tissue, HeLa cells, rat spleen tissue, rat pancreas tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15442-1-AP targets GMDS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMDS fusion protein Ag7319
Full Name GDP-mannose 4,6-dehydratase
Calculated molecular weight 42 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC000117
Gene symbol GMDS
Gene ID (NCBI) 2762
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDP-D-mannose-4,6-dehydratase (GMDS) is the key enzyme in the ‘de novo’ pathway of GDP-L-fucose biosynthesis(PMID:10462046). The enzyme is also named as GMD and belongs to the GDP-mannose 4,6-dehydratase family. The gene encodes a 42 kDa protein that catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor.

Protocols

Product Specific Protocols
WB protocol for GMDS antibody 15442-1-APDownload protocol
IHC protocol for GMDS antibody 15442-1-APDownload protocol
IF protocol for GMDS antibody 15442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Chem Biol

High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery.

Authors - Yasuhiko Kizuka
humanWB

Mol Cell Proteomics

Glycomic Profiling Highlights Increased Fucosylation in Pseudomyxoma Peritonei.

Authors - Lilli Saarinen
humanWB,IP

Mol Cell Biol

GDP-mannose-4,6-dehydratase is a cytosolic partner of tankyrase 1 that inhibits its poly(ADP-ribose) polymerase activity.

Authors - Bisht Kamlesh K KK