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  • Featured Product
  • KD/KO Validated

GMF Beta Polyclonal antibody

GMF Beta Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10690-1-AP

Synonyms

Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB, GMF-β

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMF Beta Polyclonal antibody (10690-1-AP)

    WB analysis using 10690-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF Beta antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GMF Beta Polyclonal antibody (10690-1-AP)

    WB analysis using 10690-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF Beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using GMF Beta Polyclonal antibody (10690-1-AP)

    WB analysis of mouse heart using 10690-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using GMF Beta Polyclonal antibody (10690-1-AP)

    WB analysis of human heart using 10690-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:30 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GMF Beta Polyclonal antibody (10690-1-AP)

    WB analysis of human brain using 10690-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10690-1-AP (GMF-beta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GMF Beta Polyclonal antibody (10690-1-AP)

    IHC staining of mouse brain using 10690-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10690-1-AP (GMF-beta antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GMF Beta Polyclonal antibody (10690-1-AP)

    IHC staining of mouse brain using 10690-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10690-1-AP (GMF-beta antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using GMF Beta Polyclonal antibody (10690-1-AP)

    IF Staining of mouse brain using 10690-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10690-1-AP (GMF-beta antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of U-87 MG cells using GMF Beta Polyclonal antibody (10690-1-AP)

    FC experiment of U-87 MG using 10690-1-AP

    1X10^6 U-87 MG cells were intracellularly stained with 0.4 ug Anti-Human GMF Beta (10690-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, human brain tissue, PC-12 cells, mouse heart tissue, rat brain tissue, SH-SY5Y cells, U-87 MG cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inU-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10690-1-AP targets GMF Beta in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMF Beta fusion protein Ag1104
Full Name glia maturation factor, beta
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC005359
Gene Symbol GMFB
Gene ID (NCBI) 2764
RRIDAB_2110837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon. GMFB was positive in the cytoplasm and nuclei of all cells in whole brain.

Protocols

Product Specific Protocols
WB protocol for GMF Beta antibody 10690-1-APDownload protocol
IHC protocol for GMF Beta antibody 10690-1-APDownload protocol
IF protocol for GMF Beta antibody 10690-1-APDownload protocol
FC protocol for GMF Beta antibody 10690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Development

Coronary veins determine the pattern of sympathetic innervation in the developing heart.

Authors - Nam Joseph J
View Article
humanIHC

Int J Mol Sci

Parkinson's Disease Dementia Patients: Expression of Glia Maturation Factor in the Brain

Authors - Ramasamy Thangavel
View Article
humanWB,IHC

Eur J Cancer

Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.

Authors - Li Yan Li YL
View Article
humanWB

Front Oncol

Glia Maturation Factor Beta as a Novel Biomarker and Therapeutic Target for Hepatocellular Carcinoma.

Authors - Wan Sun
View Article
humanWB,IF

Front Oncol

Glia Maturation Factor β as a Novel Independent Prognostic Biomarker and Potential Therapeutic Target of Kidney Renal Clear Cell Carcinoma.

Authors - Tong Zhu
View Article
mouse,zebrafishWB,IF

Exp Neurol

Glia maturation factor beta is required for reactive gliosis after traumatic brain injury in zebrafish.

Authors - Guo Yin
  • KO Validated
View Article