GMF-beta Antibody

GMF-beta Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10690-1-AP

Synonyms

Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB, GMF-β



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, human heart tissue, human brain tissue, mouse heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10690-1-AP targets GMF-beta in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMF-beta fusion protein Ag1104
Full Name glia maturation factor, beta
Calculated molecular weight 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC005359
Gene symbol GMFB
Gene ID (NCBI) 2764
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMFB, Glia maturation factor beta, is a nerve growth factor implicated in nervous system development. It was reported that GMF-beta promotes the phenotypic expression of glia and neurons and inhibits the proliferation of their respective tumors in culture cells. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. Higher levels of GMFB were found in the central nervous system, except for the spinal cord, and in thymus and colon. GMFB was positive in the cytoplasm and nuclei of all cells in whole brain.

Protocols

Product Specific Protocols
WB protocol for GMF-beta antibody 10690-1-APDownload protocol
IHC protocol for GMF-beta antibody 10690-1-APDownload protocol
IF protocol for GMF-beta antibody 10690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB,IF

PLoS One

Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins α-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators.

Authors - Duraisamy Kempuraj
humanIF

J Alzheimers Dis

Co-Localization of Glia Maturation Factor with NLRP3 Inflammasome and Autophagosome Markers in Human Alzheimer's Disease Brain.

Authors - Mohammad Ejaz Ahmed
humanWB,IHC

Eur J Cancer

Identification of glia maturation factor beta as an independent prognostic predictor for serous ovarian cancer.

Authors - Li Yan Li YL
mouseIHC

Development

Coronary veins determine the pattern of sympathetic innervation in the developing heart.

Authors - Nam Joseph J