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  • KD/KO Validated

GNPDA1 Polyclonal antibody

GNPDA1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12312-1-AP

Synonyms

GlcN6P deaminase 1, GNPDA, GNPDA 1, GNPDA1, GNPI, GPI, HLN, KIAA0060, Oscillin



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, human brain tissue, rat kidney tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12312-1-AP targets GNPDA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNPDA1 fusion protein Ag2961
Full Name glucosamine-6-phosphate deaminase 1
Calculated Molecular Weight 289 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC012853
Gene Symbol GNPDA1
Gene ID (NCBI) 10007
RRIDAB_2110553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNPDA1(glucosamine-6-phosphate isomerase 1) is also named as GNPI, HLN, KIAA0060 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium(PMID:12965206). It may be playing an essential role for egg activation and early embryo development. GNPI is expressed as a 33-kD cytosolic protein in various cell lines(PMID: 9714720).

Protocols

Product Specific Protocols
WB protocol for GNPDA1 antibody 12312-1-APDownload protocol
IHC protocol for GNPDA1 antibody 12312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.

Authors - Yajuan Zhao
humanWB

Cancer Manag Res

Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma.

Authors - Dezhi Li
  • KD Validated
humanWB

Glycobiology

Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.

Authors - Sanna Oikari
  • KD Validated