|Positive WB detected in||K-562 cells, HeLa cells, human brain tissue, rat kidney tissue|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12312-1-AP targets GNPDA1 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GNPDA1 fusion protein Ag2961|
|Full Name||glucosamine-6-phosphate deaminase 1|
|Calculated molecular weight||289 aa, 33 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC012853|
|Gene ID (NCBI)||10007|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GNPDA1(glucosamine-6-phosphate isomerase 1) is also named as GNPI, HLN, KIAA0060 and belongs to the glucosamine/galactosamine-6-phosphate isomerase family. It catalyzes the reversible conversion of D-glucosamine-6-phosphate (GlcN6P) into D-fructose-6-phosphate (Fru6P) and ammonium(PMID:12965206). It may be playing an essential role for egg activation and early embryo development. GNPI is expressed as a 33-kD cytosolic protein in various cell lines(PMID: 9714720).
Cancer Manag Res
Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma.
Hexosamine biosynthesis in keratinocytes: roles of GFAT and GNPDA enzymes in the maintenance of UDP-GlcNAc content and hyaluronan synthesis.