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  • KD/KO Validated

GP73/GOLPH2 Polyclonal antibody

GP73/GOLPH2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15126-1-AP

Synonyms

golgi protein 73kD, GOLM1, GOLPH2, GP73



Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, MDA-MB-231 cells, HuH-7 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, human liver cancer tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

15126-1-AP targets GP73/GOLPH2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GP73/GOLPH2 fusion protein Ag7207
Full Name golgi membrane protein 1
Calculated molecular weight 45 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC001740
Gene symbol GOLM1
Gene ID (NCBI) 51280
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IHC protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IF protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IP protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
FC protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Curr Alzheimer Res

RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase.

Authors - T Wang
humanIHC

Diagn Pathol

Expression and prognostic significance of Golgiglycoprotein73 (GP73) with Epithelial-mesenchymal transition (EMT) related molecules in Hepatocellular Carcinoma (HCC).

Authors - Bao Yong Xing YX
humanWB,IF

Biochem Biophys Res Commun

Golgi protein 73 facilitates the interaction of hepatitis C virus NS5A with apolipoprotein E to promote viral particle secretion.

Authors - Xuewu Zhang
  • KD Validated
humanWB

Eur Rev Med Pharmacol Sci

LncRNA MALAT1 knockdown inhibits cell migration and invasion by suppressing autophagy through miR-384/GOLM1 axis in glioma.

Authors - R Ma
humanWB,IHC,IF

J Cancer

GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.

Authors - Jia-Zhou Ye
  • KD Validated
humanWB

PLoS Pathog

GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.

Authors - Xuewu Zhang
  • KD Validated