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- KD/KO Validated
GP73/GOLPH2 Polyclonal antibody
GP73/GOLPH2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 15126-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, MDA-MB-231 cells, HuH-7 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, human liver cancer tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
15126-1-AP targets GP73/GOLPH2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GP73/GOLPH2 fusion protein Ag7207 |
| Full Name | golgi membrane protein 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC001740 |
| Gene symbol | GOLM1 |
| Gene ID (NCBI) | 51280 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IHC protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IF protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IP protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| FC protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Curr Alzheimer Res RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase. | ||
Diagn Pathol Expression and prognostic significance of Golgiglycoprotein73 (GP73) with Epithelial-mesenchymal transition (EMT) related molecules in Hepatocellular Carcinoma (HCC). | ||
Biochem Biophys Res Commun Golgi protein 73 facilitates the interaction of hepatitis C virus NS5A with apolipoprotein E to promote viral particle secretion.
| ||
Eur Rev Med Pharmacol Sci LncRNA MALAT1 knockdown inhibits cell migration and invasion by suppressing autophagy through miR-384/GOLM1 axis in glioma. | ||
J Cancer GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.
| ||
PLoS Pathog GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.
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