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GP73/GOLPH2 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 15126-1-AP

Featured Product KD/KO validated

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Con: 49 μg/150 μl

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Tested Applications
Applications:
ELISA, FC, IF, IHC, IP, WB
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GP73/GOLPH2 Antibody IF human liver cancer tissue 15126-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human liver cancer tissue using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

GP73/GOLPH2 Antibody IHC human liver cancer tissue 15126-1-AP

Immunohistochemical of paraffin-embedded human liver cancer using 15126-1-AP(GP73/GOLPH2 antibody) at dilution of 1:100 (under 10x lens)

GP73/GOLPH2 Antibody IF Hela cells 15126-1-AP

Immunofluorescent analysis of Hela cells, using GOLM1 antibody 15126-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

GP73/GOLPH2 Antibody IHC human liver cancer tissue 15126-1-AP

Immunohistochemical of paraffin-embedded human liver cancer using 15126-1-AP(GP73/GOLPH2 antibody) at dilution of 1:100 (under 40x lens)

GP73/GOLPH2 Antibody WB HeLa cells 15126-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15126-1-AP(GP73/GOLPH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

GP73/GOLPH2 Antibody IP HeLa cells 15126-1-AP

IP Result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

GP73/GOLPH2 Antibody FC HeLa cells 15126-1-AP

1X10^6 HeLa cells were stained with 0.2ug GP73/GOLPH2 antibody (15126-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human liver cancer tissue, human heart tissue, human kidney tissue, human liver cancer tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Positive IF detected in:
human liver cancer tissue,HeLa cells, Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GP73/GOLPH2 fusion protein Ag7207
Full name:
golgi membrane protein 1
Calculated molecular weight:
45 kDa
Observed molecular weight:
73 kDa
GenBank accession number:
BC001740
Gene ID (NCBI):
51280
Gene symbol
GOLM1
Synonyms
golgi protein 73kD, GOLM1, GOLPH2, GP73
Background

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma.


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