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GP73/GOLPH2 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 15126-1-AP

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Con: 49 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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GP73/GOLPH2 Antibody IF human liver cancer tissue 15126-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human liver cancer tissue using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

GP73/GOLPH2 Antibody IHC human liver cancer tissue 15126-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 15126-1-AP(GP73/GOLPH2 antibody) at dilution of 1:100 (under 10x lens)

GP73/GOLPH2 Antibody IF Hela cells 15126-1-AP

Immunofluorescent analysis of Hela cells, using GOLM1 antibody 15126-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

GP73/GOLPH2 Antibody IHC human liver cancer tissue 15126-1-AP

Immunohistochemical analysis of paraffin-embedded human liver cancer using 15126-1-AP(GP73/GOLPH2 antibody) at dilution of 1:100 (under 40x lens)

GP73/GOLPH2 Antibody WB HeLa cells 15126-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 15126-1-AP(GP73/GOLPH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

GP73/GOLPH2 Antibody IP HeLa cells 15126-1-AP

IP Result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

GP73/GOLPH2 Antibody FC HeLa cells 15126-1-AP

1X10^6 HeLa cells were stained with 0.2ug GP73/GOLPH2 antibody (15126-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human liver cancer tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human liver cancer tissue, HeLa cells, Hela cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GP73/GOLPH2 fusion protein Ag7207
Full name:
golgi membrane protein 1
Calculated molecular weight:
45 kDa
Observed molecular weight:
73 kDa
GenBank accession number:
BC001740
Gene ID (NCBI):
51280
Gene symbol
GOLM1
Synonyms
golgi protein 73kD, GOLM1, GOLPH2, GP73

Background

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.


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