Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15126-1-AP targets GP73/GOLPH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7207 Product name: Recombinant human GOLM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-400 aa of BC001740 Sequence: SRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL Predict reactive species |
| Full Name | golgi membrane protein 1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC001740 |
| Gene Symbol | GP73 |
| Gene ID (NCBI) | 51280 |
| RRID | AB_2113330 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBJ4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IHC protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| IP protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| WB protocol for GP73/GOLPH2 antibody 15126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-GP73 (catalog no. 15126-1-AP) has accumulated 23 citations across journals including Molecular Cancer, Molecular Cell, Developmental Cell, Journal of Immunotherapy of Cancer, PLoS Pathogens, Clinical and Molecular Hepatology, Journal of Advanced Research, Cell Signaling, Journal of Cellular Science, International Journal of Cardiology, Diagnostic Pathology, Journal of Cancer, Pediatric Research, and Current Alzheimer's Research. Research fields span hepatocellular carcinoma, colorectal cancer, ferroptosis, viral hepatitis, cardiovascular disease, glioma, epilepsy, and Alzheimer's disease.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis. | ||
Mol Cancer GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis | ||
Clin Mol Hepatol GOLM1 promotes cholesterol gallstone formation via ABCG5-mediated cholesterol efflux in MASH livers
| ||
J Extracell Vesicles A Unique Intercellular Feedforward Loop From HK1 to TGF-β1 Promotes the Progression of Hepatocellular Carcinoma. | ||
J Adv Res The GOLM1-ACLY pathway regulates macrophage-secreted EFEMP1 via H3K27ac modifications to drive tumor progression | ||
