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GOPC Polyclonal antibody

GOPC Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12163-1-AP

Synonyms

CAL, CFTR associated ligand, FIG, Fused in glioblastoma, GOPC, GOPC1, PIST

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOPC Polyclonal antibody (12163-1-AP)

    WB analysis using 12163-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12163-1-AP (GOPC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GOPC Polyclonal antibody (12163-1-AP)

    IHC staining of human pancreas cancer using 12163-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12163-1-AP (GOPC antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293T cells using GOPC Polyclonal antibody (12163-1-AP)

    IF Staining of HEK-293T using 12163-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using GOPC antibody (12163-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GOPC Polyclonal antibody (12163-1-AP)

    IF Staining of HEK-293 using 12163-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOPC antibody (12163-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, PC-3 cells, mouse colon
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293T cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12163-1-AP targets GOPC in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOPC fusion protein Ag2804
Full Name golgi associated PDZ and coiled-coil motif containing
Calculated Molecular Weight 454 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC009553
Gene Symbol GOPC
Gene ID (NCBI) 57120
RRIDAB_2113182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOPC (FIG/PIST/CAL) is a PDZ-domain scaffolding protein that regulates the trafficking of many integral membrane proteins, including cell surface receptors, ion channels and adhesion molecules. GOPC localizes to the trans-Golgi network (TGN) but not to the cis- or trans-Golgi cisternae, thus anti-GOPC can be used to label the TGN structure.

Protocols

Product Specific Protocols
WB protocol for GOPC antibody 12163-1-APDownload protocol
IHC protocol for GOPC antibody 12163-1-APDownload protocol
IF protocol for GOPC antibody 12163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Environ Int

Repression of autophagy leads to acrosome biogenesis disruption caused by a sub-chronic oral administration of polystyrene nanoparticles.

Authors - Lixiao Zhou
View Article