GOPC Antibody

GOPC Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12163-1-AP

Synonyms

CAL, CFTR associated ligand, FIG, Fused in glioblastoma, GOPC, GOPC1, PIST



Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12163-1-AP targets GOPC in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOPC fusion protein Ag2804
Full Name golgi associated PDZ and coiled-coil motif containing
Calculated molecular weight 454aa,50 kDa
Observed molecular weight 50 kDa
GenBank accession number BC009553
Gene symbol GOPC
Gene ID (NCBI) 57120
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOPC (FIG/PIST/CAL) is a PDZ-domain scaffolding protein that regulates the trafficking of many integral membrane proteins, including cell surface receptors, ion channels and adhesion molecules. GOPC localizes to the trans-Golgi network (TGN) but not to the cis- or trans-Golgi cisternae, thus anti-GOPC can be used to label the TGN structure.

Protocols

Product Specific Protocols
WB protocol for GOPC antibody 12163-1-APDownload protocol
IHC protocol for GOPC antibody 12163-1-APDownload protocol
IF protocol for GOPC antibody 12163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols