GOT1 Monoclonal antibody

GOT1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, chicken

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

5A12E10

Cat no : 60317-1-Ig

Synonyms

CAT, GIG18, GOT1, Transaminase A



Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue, rat brain tissue, rat heart tissue, K-562 cells, chicken brain, chicken heart
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

60317-1-Ig targets GOT1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

Tested Reactivity human, mouse, rat, chicken
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOT1 fusion protein Ag7012
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Calculated molecular weight 46 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC000498
Gene symbol GOT1
Gene ID (NCBI) 2805
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.

Protocols

Product Specific Protocols
WB protocol for GOT1 antibody 60317-1-IgDownload protocol
IHC protocol for GOT1 antibody 60317-1-IgDownload protocol
IF protocol for GOT1 antibody 60317-1-IgDownload protocol
FC protocol for GOT1 antibody 60317-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Authors - Wenzhu Li