|Positive WB detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:3200|
|Sample-dependent, check data in validation data gallery|
14820-1-AP targets GPR37/Pael-R in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GPR37/Pael-R fusion protein Ag6591|
|Full Name||G protein-coupled receptor 37 (endothelin receptor type B-like)|
|Calculated molecular weight||67 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC040007|
|Gene ID (NCBI)||2861|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).
J Biol Chem
GPR37 Trafficking to the Plasma Membrane Regulated by Prosaposin and GM1 Gangliosides Promotes Cell Viability.
Glio- and neuro-protection by prosaposin is mediated by orphan G-protein coupled receptors GPR37L1 and GPR37.
Parkinson's disease-associated receptor GPR37 is an ER chaperone for LRP6.
Proc Natl Acad Sci U S A
GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.
Mol Syst Biol
Systematic protein-protein interaction mapping for clinically relevant human GPCRs.