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  • KD/KO Validated

GPR37/Pael-R Polyclonal antibody

GPR37/Pael-R Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 14820-1-AP

Synonyms

EDNRBL, ETBR LP 1, GPR37, hET(B)R LP, PAELR, Pael-R



Tested Applications

Positive WB detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3200
Sample-dependent, check data in validation data gallery

Product Information

14820-1-AP targets GPR37/Pael-R in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR37/Pael-R fusion protein Ag6591
Full Name G protein-coupled receptor 37 (endothelin receptor type B-like)
Calculated molecular weight 67 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC040007
Gene symbol GPR37
Gene ID (NCBI) 2861
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).

Protocols

Product Specific Protocols
WB protocol for GPR37/Pael-R antibody 14820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

J Biol Chem

GPR37 Trafficking to the Plasma Membrane Regulated by Prosaposin and GM1 Gangliosides Promotes Cell Viability.

Authors - Ebba Gregorsson Lundius
ratWB

Glia

Glio- and neuro-protection by prosaposin is mediated by orphan G-protein coupled receptors GPR37L1 and GPR37.

Authors - Beihui Liu
humanWB

EMBO Rep

Parkinson's disease-associated receptor GPR37 is an ER chaperone for LRP6.

Authors - Birgit S Berger
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin.

Authors - Meyer Rebecca C RC
humanWB

Mol Syst Biol

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.

Authors - Kate Sokolina