GRIP1 Polyclonal antibody

GRIP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22398-1-AP

Synonyms

GRIP, GRIP1



Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22398-1-AP is GRIP1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRIP1 fusion protein Ag18071
Full Name glutamate receptor interacting protein 1
Calculated molecular weight 1128 aa, 122 kDa
Observed molecular weight 130-135 kDa
GenBank accession numberBC115393
Gene symbol GRIP1
Gene ID (NCBI) 23426
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRIP1 antibody 22398-1-APDownload protocol
IHC protocol for GRIP1 antibody 22398-1-APDownload protocol
IF protocol for GRIP1 antibody 22398-1-APDownload protocol
IP protocol for GRIP1 antibody 22398-1-APDownload protocol
FC protocol for GRIP1 antibody 22398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neuropharmacology

Regulation of hippocampal long term depression by Neuroligin 1.

Authors - Rui Dang