Tested Applications
| Positive WB detected in | MCF-7 cells, U2Os cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85907-4-RR targets GRK6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag1992 Product name: Recombinant human GRK6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 54-451 aa of BC017272 Sequence: HSLCERQPIGRLLFREFCATRPELSRCVAFLDGVAEYEVTPDDKRKACGRQLTQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCFLYEMIAGQSPFQQRKKKIKREEVERLVKEVPEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKKL Predict reactive species |
| Full Name | G protein-coupled receptor kinase 6 |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC017272 |
| Gene Symbol | GRK6 |
| Gene ID (NCBI) | 2870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43250 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GRK6 (G Protein-Coupled Receptor Kinase 6) is a member of the G protein-coupled receptor kinase (GRK) family, which plays a crucial role in regulating the activity of G protein-coupled receptors (GPCRs). GRK6 specifically phosphorylates the activated forms of GPCRs, initiating their desensitization, internalization, and signaling events that lead to receptor deactivation. This process is essential for maintaining proper cellular responses to extracellular signals.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRK6 antibody 85907-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
