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GSK3B Polyclonal antibody

GSK3B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15113-1-AP

Synonyms

GSK 3 beta, GSK3 beta, GSK3A B, GSK3B, GSK3B 216P, gsk3beta



Tested Applications

Positive WB detected inmouse ovary tissue, HEK-293 cells, HeLa cells, A549 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

15113-1-AP targets GSK3B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSK3B fusion protein Ag7101
Full Name glycogen synthase kinase 3 beta
Calculated molecular weight 433 aa, 48 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000251
Gene symbol GSK3B
Gene ID (NCBI) 2932
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .This antibody recognize the 2 isoforms of GSK3B.

Protocols

Product Specific Protocols
WB protocol for GSK3B antibody 15113-1-APDownload protocol
IHC protocol for GSK3B antibody 15113-1-APDownload protocol
IP protocol for GSK3B antibody 15113-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Biol Int

Identification and analysis of glycogen synthase kinase 3 beta1 interactome.

Authors - Gao Xuejuan X
humanWB

J Ethnopharmacol

Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway.

Authors - Tao Zheng