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GTF2IRD1 Polyclonal antibody

GTF2IRD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17052-1-AP

Synonyms

BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    WB analysis of HeLa using 17052-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    WB analysis of mouse liver using 17052-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    WB analysis of mouse brain using 17052-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    WB analysis of mouse kidney using 17052-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IP experiment of mouse liver using 17052-1-AP

    IP result of anti-GTF2IRD1 (IP:17052-1-AP, 4ug; Detection:17052-1-AP 1:500) with mouse liver tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human testis using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human skin using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human skin using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human brain using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human brain using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human liver using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human liver using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human spleen using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human spleen using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human ovary using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human ovary using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human heart using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human heart using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human kidney using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human kidney using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IHC staining of human testis using 17052-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GTF2IRD1 Polyclonal antibody (17052-1-AP)

    IF Staining of HeLa using 17052-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman testis tissue, human spleen tissue, human kidney tissue, human heart tissue, human ovary tissue, human skin tissue, human brain tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17052-1-AP targets GTF2IRD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2IRD1 fusion protein Ag10718
Full Name GTF2I repeat domain containing 1
Calculated Molecular Weight 959 aa, 106 kDa
Observed Molecular Weight 145 kDa, 120 kDa
GenBank Accession NumberBC018136
Gene Symbol GTF2IRD1
Gene ID (NCBI) 9569
RRIDAB_2114956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IHC protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IF protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
IP protocol for GTF2IRD1 antibody 17052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols