Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 17052-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17052-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 17052-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 17052-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 17052-1-AP
IP result of anti-GTF2IRD1 (IP:17052-1-AP, 4ug; Detection:17052-1-AP 1:500) with mouse liver tissue lysate 4000ug.
IHC staining of human testis using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human kidney using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 17052-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 17052-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17052-1-AP (GTF2IRD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GTF2IRD1 Polyclonal antibody

Name: GTF2IRD1 Polyclonal antibody
Brand: Proteintech
SKU: 17052-1-AP

GTF2IRD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17052-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BEN, CREAM1, GTF2IRD1, GTF3, hMusTRD1alpha1, MUSTRD1, MusTRD1/BEN, RBAP2, USE B1 binding protein, WBS, WBSCR11, WBSCR12

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human testis tissue, human spleen tissue, human kidney tissue, human heart tissue, human ovary tissue, human skin tissue, human brain tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

17052-1-AP targets GTF2IRD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10718 Product name: Recombinant human GTF2IRD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 610-959 aa of BC018136 Sequence: ISLRRPNCFGIAKLRKILEASNSIQFVIKRPELLTEGVKEPIVDSQGTASSLGFSPPALPPERDSGDPLVDESLKRQGFQENYDARLSRIDIANTLREQVQDLFNKKYGEALGIKYPVQVPYKRIKSNPGSVIIEGLPPGIPFRKPCTFGSQNLERILAVADKIKFTVTRPFQGLIPKPDEDDANRLGEKVILREQVKELFNEKYGEALGLNRPVLVPYKLIRDSPDAVEVTGLPDDIPFRNPNTYDIHRLEKILKAREHVRMVIINQLQPFAEICNDAKVPAKDSSIPKRKRKRVSEGNSVSSSSSSSSSSSSNPDSVASANQISLVQWPMYMVDYAGLNVQLPGPLNY Predict reactive species
Full Name	GTF2I repeat domain containing 1
Calculated Molecular Weight	959 aa, 106 kDa
Observed Molecular Weight	145 kDa, 120 kDa
GenBank Accession Number	BC018136
Gene Symbol	GTF2IRD1
Gene ID (NCBI)	9569
RRID	AB_2114956
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UHL9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol
IHC protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol
IP protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol
WB protocol for GTF2IRD1 antibody 17052-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Int J Surg Single-cell and spatial transcriptomic analyses reveal heterogeneity characteristics and specific cell subtype regulators in growth hormone-secreting pituitary adenomas Authors - Yu Zhang View Article