Tested Applications
| Positive WB detected in | Daudi cells, PC-3 cells, pig thymus tissue, ROS1728 cells, human heart tissue, RAW 264.7 cells, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60355-1-Ig targets TIM3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16901 Product name: Recombinant human HAVCR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-142 aa of BC020843 Sequence: MFSHLPFDCVLLLLLLLLTRSSEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPGEWTFACHLYE Predict reactive species |
| Full Name | hepatitis A virus cellular receptor 2 |
| Calculated Molecular Weight | 301 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa, 50-70 kDa |
| GenBank Accession Number | BC020843 |
| Gene Symbol | TIM3 |
| Gene ID (NCBI) | 84868 |
| RRID | AB_2881464 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q8TDQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TIM3 antibody 60355-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-TIM-3 (Cat# 60355-1-Ig) has 31 citations across journals including Cancer Cell, Nature Communications, Advanced Science, Oncogene, Cell Death & Disease, Frontiers in Immunology, EBioMedicine, Small, and iScience. Research fields span cancer immunology (glioblastoma, breast cancer, gastric cancer, melanoma, prostate cancer, DLBCL), immune checkpoint biology, diabetic nephropathy, pregnancy immunology, autoimmune diseases (dermatomyositis, ankylosing spondylitis), and tissue repair.
| Species | Application | Title |
|---|---|---|
Cancer Cell TIM3+ breast cancer cells license immune evasion during micrometastasis outbreak | ||
Nat Commun Redox-responsive polymer micelles co-encapsulating immune checkpoint inhibitors and chemotherapeutic agents for glioblastoma therapy | ||
Adv Sci (Weinh) Dysfunctional Decidual CD4+T Cells Induce Recurrent Pregnancy Loss via Palmitoylation-Dependent Tim-3 Lysosomal Sorting and Degradation | ||
Cell Death Dis Helicobacter pylori CagA promotes gastric cancer immune escape by upregulating SQLE | ||
Small Engineered Exosome-Loaded Silk Fibroin Composite Hydrogels Promote Tissue Repair in Spinal Cord Injury Via Immune Checkpoint Blockade | ||
EBioMedicine Immunological profiles of human oligodendrogliomas define two distinct molecular subtypes |
Reviews
What customers say
The single review for this product (lot 10002413) was posted in April 2019 by a user named Santhosh, who rated it 4 out of 5 stars. The reviewer used the antibody at a 1:200 dilution for immunohistochemistry on human tonsil FFPE tissue, employing heat-mediated antigen retrieval at pH 6. Overall, the feedback suggests satisfactory performance in IHC applications on human tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Santhosh (Verified Customer) (04-15-2019) | Human Tonsil FFPE tissue. Heat mediated antigen retrieval (pH 6)
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