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  • KD/KO Validated

TIM3 Monoclonal antibody

TIM3 Monoclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

4C4G3

Cat no : 60355-1-Ig

Synonyms

CD366, HAVcr 2, HAVCR2, KIM 3, T cell membrane protein 3, Tim 3, TIM3, TIM-3, TIMD 3, TIMD3



Tested Applications

Positive WB detected inDaudi cells, HEK-293 cells, PC-3 cells, Jurkat cells, pig thymus tissue, ROS1728 cells, human heart tissue, RAW 264.7 cells, rat heart tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inDaudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60355-1-Ig targets TIM3 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TIM3 fusion protein Ag16901
Full Name hepatitis A virus cellular receptor 2
Calculated molecular weight 301 aa, 33 kDa
Observed molecular weight 33 kDa, 50-70 kDa
GenBank accession numberBC020843
Gene symbol HAVCR2
Gene ID (NCBI) 84868
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TIM3, also known as HAVCR2, is a member of the recently discovered T cell Ig and mucin domain-containing molecule superfamily. TIM3 is a negative regulatory molecule that is important for T cell tolerance and has a crucial role in autoimmunity and T cell exhaustion during chronic viral infection (PMID: 23180819). TIM3 is expressed by T-helper type 1 (Th1) cells, macrophage, monocyte, dendritic cells, CD8+ T cell and other lymphocyte subsets. Galectin-9 is a ligand for TIM3. TIM3-galectin-9 pathway negatively regulates T helper type 1 immunity (PMID: 16286920). TIM3 is a 280-aa membrane protein with a calculated molecular weight of 33 kDa, the higher molecular weights between 50 and 70 kDa detected by this monoclonal antibody probably represent glycosylated TIM3 (PMID: 20107545; 17069754; 11725301).

Protocols

Product Specific Protocols
WB protocol for TIM3 antibody 60355-1-IgDownload protocol
IHC protocol for TIM3 antibody 60355-1-IgDownload protocol
FC protocol for TIM3 antibody 60355-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Mol Metab

Tim-3 aggravates podocyte injury in diabetic nephropathy by promoting macrophage activation via the NF-κB/TNF-α pathway.

Authors - Huimin Yang
mouseIF

Mol Med Rep

Tim‑3 regulates the ability of macrophages to counter lipopolysaccharide‑induced pulmonary epithelial barrier dysfunction via the PI3K/Akt pathway in epithelial cells.

Authors - Yuntao Zhang
mouseIHC

Scand J Immunol

Cross-regulation by TLR4 and T cell Ig mucin-3 determines severity of liver injury in a CCl4-induced mouse model.

Authors - Lizhen Zhao
humanIHC

Sci Rep

Low TIM3 expression indicates poor prognosis of metastatic prostate cancer and acts as an independent predictor of castration resistant status.

Authors - Junlong Wu
mouseWB

Front Immunol

Role of Tim-3 in Decidual Macrophage Functional Polarization During Abnormal Pregnancy With Toxoplasma gondii Infection.

Authors - Dan Zhang
  • KO Validated
humanIHC

J Pathol

Molecular subtyping reveals immune alterations in IDH wild-type lower-grade diffuse glioma.

Authors - Fan Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Santhosh (Verified Customer) (04-15-2019)

Human Tonsil FFPE tissue. Heat mediated antigen retrieval (pH 6)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:200
TIM3 Antibody Immunohistochemistry, validation (1:200 dilution) in (Cat no:60355-1-Ig)