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HCCS Polyclonal antibody

HCCS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15118-1-AP

Synonyms

CCHL, Cytochrome c type heme lyase, HCCS, Holocytochrome c type synthase, MCOPS7

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using HCCS Polyclonal antibody (15118-1-AP)

    WB analysis of human heart using 15118-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using HCCS Polyclonal antibody (15118-1-AP)

    WB analysis of MCF-7 using 15118-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HCCS Polyclonal antibody (15118-1-AP)

    WB analysis of HeLa using 15118-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15118-1-AP (HCCS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using HCCS Polyclonal antibody (15118-1-AP)

    IP experiment of MCF-7 using 15118-1-AP

    IP result of anti-HCCS (IP:15118-1-AP, 4ug; Detection:15118-1-AP 1:500) with MCF-7 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HCCS Polyclonal antibody (15118-1-AP)

    IHC staining of human breast cancer using 15118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HCCS Polyclonal antibody (15118-1-AP)

    IHC staining of human breast cancer using 15118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HCCS Polyclonal antibody (15118-1-AP)

    IHC staining of human breast cancer using 15118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HCCS Polyclonal antibody (15118-1-AP)

    IHC staining of human breast cancer using 15118-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15118-1-AP (HCCS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HCCS Polyclonal antibody (15118-1-AP)

    IF Staining of HeLa using 15118-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15118-1-AP (HCCS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman heart tissue, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15118-1-AP targets HCCS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HCCS fusion protein Ag7189
Full Name holocytochrome c synthase (cytochrome c heme-lyase)
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC001691
Gene Symbol HCCS
Gene ID (NCBI) 3052
RRIDAB_2114848
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HCCS(Cytochrome c-type heme lyase) links covalently the heme group to the apoprotein of cytochrome C.The covalent attachment of heme by CCHL in the IMS is crucial for translocation of the precursor of cytochrome c and its trapping in the IMS(PMID:15359280 ).This protein has a calculated molecular mass of about 31 KDa.

Protocols

Product Specific Protocols
WB protocol for HCCS antibody 15118-1-APDownload protocol
IHC protocol for HCCS antibody 15118-1-APDownload protocol
IF protocol for HCCS antibody 15118-1-APDownload protocol
IP protocol for HCCS antibody 15118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Mol Med

SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.

Authors - Alexandre Janer
View Article
humanWB

Chem Pharm Bull (Tokyo)

The Novel Synthetic Triterpene Methyl 3β-O-[4-(2-Aminoethylamino)-4-oxo-butyryl]olean-12-ene-28-oate Inhibits Breast Tumor Cell Growth in Vitro and in Vivo.

Authors - Bin Feng
View Article
human,mouseWB

Nat Cell Biol

Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space

Authors - Elias Adriaenssens
View Article