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HDAC1 Antibody 16 Publications

Rabbit Polyclonal| Catalog number: 10197-1-AP

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

HDAC1 Antibody WB HepG2 cells 10197-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

HDAC1 Antibody WB HepG2 cells 10197-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 10197-1-AP(HDAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

HDAC1 Antibody IP mouse testis tissue 10197-1-AP

IP Result of anti-HDAC1 (IP:10197-1-AP, 3ug; Detection:10197-1-AP 1:500) with mouse testis tissue lysate 10000ug.

HDAC1 Antibody IHC human pancreas cancer tissue 10197-1-AP

Immunohistochemical of paraffin-embedded human pancreas cancer using 10197-1-AP(HDAC1 antibody) at dilution of 1:50 (under 40x lens)

HDAC1 Antibody IF SH-SY5Y cells 10197-1-AP

Immunofluorescent analysis of SH-SY5Y cells, using HDAC1 antibody 10197-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).

HDAC1 Antibody FC HeLa cells 10197-1-AP

1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (10197-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, HeLa cells, mouse testis tissue
Positive IP detected in:
mouse testis tissue
Positive IHC detected in:
human pancreas cancer tissue, human testis tissue
Positive IF detected in:
SH-SY5Y cells
Positive FC detected in:
HeLa cells, SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HDAC1 fusion protein Ag0256
Full name:
histone deacetylase 1
Calculated molecular weight:
55 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC000301
Gene ID (NCBI):
3065
Gene symbol
HDAC1
Synonyms
GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

Background

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC1. The calcualted molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .


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