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HDAC1 Antibody 23 Publications

Rabbit Polyclonal| Catalog number: 10197-1-AP

Featured Product KD/KO validated

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, co-IP, ELISA
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Images See all

HDAC1 Antibody WB HepG2 cells 10197-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

HDAC1 Antibody IP mouse testis tissue 10197-1-AP

IP Result of anti-HDAC1 (IP:10197-1-AP, 3ug; Detection:10197-1-AP 1:500) with mouse testis tissue lysate 10000ug.

HDAC1 Antibody IHC human pancreas cancer tissue 10197-1-AP

Immunohistochemical of paraffin-embedded human pancreas cancer using 10197-1-AP(HDAC1 antibody) at dilution of 1:50 (under 40x lens)

HDAC1 Antibody IF SH-SY5Y cells 10197-1-AP

Immunofluorescent analysis of SH-SY5Y cells, using HDAC1 antibody 10197-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).

HDAC1 Antibody FC HeLa cells 10197-1-AP

1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (10197-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HepG2 cells, HeLa cells, mouse testis tissue
Positive IP detected in:
mouse testis tissue
Positive IHC detected in:
human pancreas cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
SH-SY5Y cells
Positive FC detected in:
HeLa cells, SH-SY5Y cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
HDAC1 fusion protein Ag0256
Full name:
histone deacetylase 1
Calculated molecular weight:
55 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC000301
Gene ID (NCBI):
3065
Gene symbol
HDAC1
Synonyms
GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

Background

HDAC1 poly https://www.ptglab.com/products/HDAC1-Antibody-10197-1-AP.htm

Background

Histone Deacetylase 1 (HDAC1) is a nuclear localized class I histone deacetylase that plays a role in the regulation of gene expression, mainly by repression of gene activity. Additionally, HDAC can mediate deacetylation of a subset of non-histone proteins, leading to their degradation. HDAC1 activity has an impact on cell growth, proliferation, and death.

 

1. What is the molecular weight of HDAC1?

The molecular size of HDAC1 is 60 kDa.

2. What is the subcellular localization of HDAC1?

Unlike some HDACs, HDAC1 is present entirely in the nucleus. Our HDAC1 antibody has been broadly tested for IF/ICC.

3. I cannot detect an HDAC1 specific signal in my sample during western blotting.

Make sure that you efficiently extract nuclear proteins during your sample preparation. Some lysis buffers (e.g., based on Triton X-100) may not extract nuclear proteins. We recommend using RIPA buffer (https://www.ptglab.com/support/protocols/). We highly recommend using nuclear loading control antibodies, such as lamin B1 or PCNA (https://www.ptglab.com/news/blog/loading-control-antibodies-for-western-blotting/). Alternatively, you may consider performing cell fractionation.

4. How do I perform chromatin immunoprecipitation (ChiP) with the HDAC1 antibody?

We recommend using our standard chromatin immunoprecipitation protocol (https://www.ptglab.com/media/2708/web_chip-protocol.pdf).

5. Is HDAC1 post-translationally modified?

HDAC1 is a protein deacetylase but itself can also be a subject of post-translational modifications, including phosphorylation, acetylation, ubiquitination, SUMOylation, nitrosylation, and carbonylation (PMID: 21197454).

6. What is the role of HDAC1 in cancer?

The acetylation and deacetylation of histones play an important part in the epigenetic regulation of gene expression. Alterations in the balance between these two opposing processes changes chromosome remodeling and increased deacetylation leads to gene silencing. HDAC1 levels are increased in many cancer cell types and the role of HDAC1 in cancer is connected not only to histone deacetylation but also to the deacetylation of other proteins, such as Rb family proteins, estrogen receptors, and p53 protein (PMID: 19383284).



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