CL594-conjugated HDAC2 Antibody

HDAC2 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A3E4

Cat no : CL594-67165

Synonyms

HD2, HDAC2, histone deacetylase 2, RPD3, YAF1



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67165-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67165 targets CL594-conjugated HDAC2 in IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HDAC2 fusion protein Ag21288
Full Name histone deacetylase 2
Calculated molecular weight 582aa,65 kDa;488aa,55 kDa
Observed molecular weight 55 kDa
GenBank accession number BC031055
Gene symbol HDAC2
Gene ID (NCBI) 3066
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

Protocols

Product Specific Protocols
IF protocol for CL594-conjugated HDAC2 antibody CL594-67165Download protocol
Standard Protocols
Click here to view our Standard Protocols