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  • KD/KO Validated

HDAC2 Monoclonal antibody

HDAC2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b


Human, mouse, rat and More (1)







Cat no : 67165-1-Ig


HD2, HDAC, HDAC2, histone deacetylase 2, RPD3, YAF1

Tested Applications

Positive WB detected inU2OS cells, 4T1 cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67165-1-Ig targets HDAC2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HDAC2 fusion protein Ag21288
Full Name histone deacetylase 2
Calculated Molecular Weight458 aa, 52 kDa; 488 aa,55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031055
Gene Symbol HDAC2
Gene ID (NCBI) 3066
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is raised against residues near the C terminus of human HDAC2.


Product Specific Protocols
WB protocol for HDAC2 antibody 67165-1-IgDownload protocol
IHC protocol for HDAC2 antibody 67165-1-IgDownload protocol
IF protocol for HDAC2 antibody 67165-1-IgDownload protocol
FC protocol for HDAC2 antibody 67165-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression

Authors - Zhilei Zhang
  • KD Validated


Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway

Authors - Xintian Lan

Toxicol Appl Pharmacol

Advanced oxidation protein products upregulate ABCB1 expression and activity via HDAC2-Foxo3α-mediated signaling in vitro and in vivo.

Authors - Tianrong Xun
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Xiaoyu (Verified Customer) (06-28-2023)

Good for wb

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T
HDAC2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:67165-1-Ig)