HLA-DQA1 Polyclonal antibody

HLA-DQA1 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 16918-1-AP

Synonyms

CELIAC1, DC 1 alpha chain, DC alpha, DQ A1, GSE, HLA DCA, HLA DQA, HLA DQA1, HLA-DQA1, MHC class II DQA1



Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16918-1-AP is HLA-DQA1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HLA-DQA1 fusion protein Ag10224
Full Name major histocompatibility complex, class II, DQ alpha 1
Calculated Molecular Weight 254 aa, 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC008585
Gene Symbol HLA-DQA1
Gene ID (NCBI) 3117
RRIDAB_2878332
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for HLA-DQA1 antibody 16918-1-APDownload protocol
IHC protocol for HLA-DQA1 antibody 16918-1-APDownload protocol
FC protocol for HLA-DQA1 antibody 16918-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

PLoS One

Variations in the MHC region confer risk to esophageal squamous cell carcinoma on the subjects from high-incidence area in northern china.

Authors - Fang-Fang Shen
humanIHC

Front Genet

Integrated Analysis of Key Genes and Pathways Involved in Fetal Growth Restriction and Their Associations With the Dysregulation of the Maternal Immune System.

Authors - Xue Wang
humanWB

Life Sci

Identification of proteomic signatures associated with COPD frequent exacerbators.

Authors - Pengbo Sun