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  • KD/KO Validated

HNRNPK Polyclonal antibody

HNRNPK Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, FC, chIP,ELISA

Conjugate

Unconjugated

Cat no : 11426-1-AP

Synonyms

CSBP, hnRNP K, HNRNPK, HNRPK, TUNP



Tested Applications

Positive WB detected inMCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11426-1-AP targets HNRNPK in WB, RIP, IP, IHC, IF, FC, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, sika deer
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HNRNPK fusion protein Ag1976
Full Name heterogeneous nuclear ribonucleoprotein K
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight 55-65 kDa
GenBank accession numberBC014980
Gene symbol HNRNPK
Gene ID (NCBI) 3190
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HNRNPK belongs to the heterogeneous nuclear ribonucleoprotein family of 20 proteins that participate in a wide range of key cellular functions involving many of the pathways implicated, disrupted or dysregulated in tumor development and progression. It is also a conserved pre-mRNA-binding protein that is involved in multiple processes of gene expression, including chromatin remodeling, transcription, and mRNA splicing, translation, and stability

Protocols

Product Specific Protocols
WB protocol for HNRNPK antibody 11426-1-APDownload protocol
IHC protocol for HNRNPK antibody 11426-1-APDownload protocol
IF protocol for HNRNPK antibody 11426-1-APDownload protocol
IP protocol for HNRNPK antibody 11426-1-APDownload protocol
FC protocol for HNRNPK antibody 11426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sika deerWB

J Proteomics

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Authors - Zhen Dong
mouseWB,chIP

Mol Cell

Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X.

Authors - David Colognori
humanWB

Cell Biol Int

Identification and analysis of glycogen synthase kinase 3 beta1 interactome.

Authors - Gao Xuejuan X
humanWB

J Mol Biol

Proteomic characterization of the nucleolar linker histone H1 interaction network.

Authors - Heather J Szerlong
humanWB

J Virol

hnRNP K is a novel ITAF that negatively regulates foot-and-mouth disease virus translation and replication and is antagonized by viral 3C protease.

Authors - Wenming Liu
humanWB

J Proteome Res

Identification of human host proteins contributing to H5N1 influenza virus propagation by membrane proteomics.

Authors - Liu Cheng C

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

DEBJIT (Verified Customer) (09-17-2020)

WB: 1:2000 IP: 2ug/mg cell lysate

  • Applications: Western Blot,Immunoprecipitation
  • Cell Tissue Type: Endothelial cells