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HOXC10 Recombinant monoclonal antibody (86219-3-RR)

HOXC10 Recombinant monoclonal antibody

HOXC10 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86219-3-RR

Clone No.250873D8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Homeobox protein Hox-3I, Homeobox protein Hox-C10, HOX3I

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Quantity

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Filter: AllWBIF/ICCAffinity

WB analysis using 86219-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86219-3-RR (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 86219-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 86219-3-RR (HOXC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 86219-3-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HOXC10 antibody (86219-3-RR, Clone: 250873D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Affinity and Kinetic Characterization of 86219-3-RR
Biolayer interferometry (BLl) kinetic assays of 86219-3-RR against Human HOXC10 were performed. The affinity constant is below 1 pM.

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	T-47D cells, U2OS cells, HeLa cells， A375 cells, C2C12 cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86219-3-RR targets HOXC10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag2655 Product name: Recombinant human HOXC10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-342 aa of BC001293 Sequence: MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTGNWLTAKSGRKKRCPYTKHQTLELEKEFLFNMYLTRERRLEISKTINLTDRQVKIWFQNRRMKLKKMNRENRIRELTSNFNFT Predict reactive species
Full Name	homeobox C10
Calculated Molecular Weight	342 aa, 38 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC001293
Gene Symbol	HOXC10
Gene ID (NCBI)	3226
RRID	AB_3744724
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9NYD6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486).