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  • KD/KO Validated

HSP90 Antibody

HSP90 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13171-1-AP

Synonyms

Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2



Tested Applications

Positive WB detected inHeLa cells, 3T3-L1 cells, C6 cells, HEK-293 cells, human liver tissue, K-562 cells, mouse kidney tissue, rat brain tissue, Y79 cells
Positive IP detected inK-562 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13171-1-AP targets HSP90 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chick, chicken, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSP90 fusion protein Ag3826
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Calculated molecular weight 853aa,90 kDa
Observed molecular weight 90 kDa
GenBank accession number BC023006
Gene symbol HSP90AA1
Gene ID (NCBI) 3320
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

Protocols

Product Specific Protocols
WB protocol for HSP90 antibody 13171-1-APDownload protocol
IHC protocol for HSP90 antibody 13171-1-APDownload protocol
IF protocol for HSP90 antibody 13171-1-APDownload protocol
IP protocol for HSP90 antibody 13171-1-APDownload protocol
FC protocol for HSP90 antibody 13171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

Chaperone-mediated autophagy is involved in the execution of ferroptosis.

Authors - Zheming Wu
humanWB

J Virol

PMID: 31092569

Authors - Dang Wang
humanWB

Cancer Med

Identification and validation of circulating exosomes-based liquid biopsy for esophageal cancer.

Authors - An Zhao
chickenWB

Int Immunopharmacol

Impacts of simultaneous exposure to arsenic (III) and copper (II) on inflammatory response, immune homeostasis, and heat shock response in chicken thymus.

Authors - Juanjuan Liu
ratWB

Acta Neurochir Suppl

17-Allylamino-Demethoxygeldanamycin Ameliorate Microthrombosis Via HSP90/RIP3/NLRP3 Pathway After Subarachnoid Hemorrhage in Rats.

Authors - Yuchun Zuo
humanWB

EBioMedicine

The LIV-1-GRPEL1 axis adjusts cell fate during anti-mitotic agent-damaged mitosis.

Authors - Pingbo Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Xiaoping (Verified Customer) (10-22-2018)

This antibody works well in iPS, iMns or MEF. The signal is pretty strong which is better than the one we used before and the background is very low.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human neuron and mouse fibroblast samples
HSP90 Antibody Western Blot, validation (1:1000 dilution) in Human neuron and mouse fibroblast samples (Cat no:13171-1-AP)